Spatially Restricted Immune Responses Are Required for Maintaining Root Meristematic Activity upon Detection of Bacteria.

Details

Serval ID
serval:BIB_588DB4B995B9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatially Restricted Immune Responses Are Required for Maintaining Root Meristematic Activity upon Detection of Bacteria.
Journal
Current biology
Author(s)
Emonet A., Zhou F., Vacheron J., Heiman C.M., Dénervaud Tendon V., Ma K.W., Schulze-Lefert P., Keel C., Geldner N.
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Publication state
Published
Issued date
08/03/2021
Peer-reviewed
Oui
Volume
31
Number
5
Pages
1012-1028.e7
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Plants restrict immune responses to vulnerable root parts. Spatially restricted responses are thought to be necessary to avoid constitutive responses to rhizosphere microbiota. To directly demonstrate the importance of spatially restricted responses, we expressed the plant flagellin receptor (FLS2) in different tissues, combined with fluorescent defense markers for immune readouts at cellular resolution. Our analysis distinguishes responses appearing cell autonomous from apparently non-cell-autonomous responses. It reveals lignification as a general immune response, contrasting suberization. Importantly, our analysis divides the root meristem into a central zone refractory to FLS2 expression and a cortex that is sensitized by FLS2 expression, causing meristem collapse upon stimulation. Meristematic epidermal expression generates super-competent lines that detect native bacterial flagellin and bypass the weak or absent response to commensals, providing a powerful tool for studying root immunity. Our manipulations and readouts demonstrate incompatibility of meristematic activity and defense and the importance of cell-resolved studies of plant immune responses.
Keywords
Arabidopsis, cell-specific response, commensal bacteria, flagellin, lignin, meristem, microbe-associated molecular pattern, pattern recognition receptor, root immune response
Pubmed
Web of science
Open Access
Yes
Create date
08/02/2021 17:00
Last modification date
17/04/2021 6:33
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