Use of an in-house approach to study the three-dimensional structures of various outer membrane proteins: structure of the alcaligin outer membrane transporter FauA from Bordetella pertussis.

Details

Serval ID
serval:BIB_5869DA0DD92E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Use of an in-house approach to study the three-dimensional structures of various outer membrane proteins: structure of the alcaligin outer membrane transporter FauA from Bordetella pertussis.
Journal
Acta Crystallographica. Section D, Biological Crystallography
Author(s)
Brillet K., Meksem A., Lauber E., Reimmann C., Cobessi D.
ISSN
1399-0047[electronic], 0907-4449[linking]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
65
Number
Pt 4
Pages
326-331
Language
english
Abstract
Bordetella pertussis is the bacterial agent of whooping cough in humans. Under iron-limiting conditions, it produces the siderophore alcaligin. Released to the extracellular environment, alcaligin chelates iron, which is then taken up as a ferric alcaligin complex via the FauA outer membrane transporter. FauA belongs to a family of TonB-dependent outer membrane transporters that function using energy derived from the proton motive force. Using an in-house protocol for membrane-protein expression, purification and crystallization, FauA was crystallized in its apo form together with three other TonB-dependent transporters from different organisms. Here, the protocol used to study FauA is described and its three-dimensional structure determined at 2.3 A resolution is discussed.
Keywords
Apoproteins/chemistry, Bacterial Outer Membrane Proteins/chemistry, Bacterial Outer Membrane Proteins/isolation & purification, Bacterial Proteins/metabolism, Bordetella pertussis/chemistry, Crystallography, X-Ray, Hydroxamic Acids/metabolism, Iron/metabolism, Membrane Proteins/metabolism, Models, Molecular, Protein Conformation, Protein Interaction Mapping, Protons, Receptors, Cell Surface/chemistry, Receptors, Cell Surface/isolation & purification, Recombinant Fusion Proteins/chemistry, Recombinant Fusion Proteins/isolation & purification
Pubmed
Web of science
Create date
02/02/2010 15:33
Last modification date
20/08/2019 14:12
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