Urinary analysis of four testosterone metabolites and pregnanediol by gas chromatography-combustion-isotope ratio mass spectrometry after oral administrations of testosterone.

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Ressource 1Download: serval:BIB_572628EF6E09.P001 (562.88 [Ko])
State: Public
Version: author
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Serval ID
serval:BIB_572628EF6E09
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Urinary analysis of four testosterone metabolites and pregnanediol by gas chromatography-combustion-isotope ratio mass spectrometry after oral administrations of testosterone.
Journal
Journal of analytical toxicology
Author(s)
Maître A., Saudan C., Mangin P., Saugy M.
ISSN
0146-4760
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
28
Number
6
Pages
426-31
Language
english
Notes
Publication types: Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
The most frequently used method to demonstrate testosterone abuse is the determination of the testosterone and epitestosterone concentration ratio (T/E ratio) in urine. Nevertheless, it is known that factors other than testosterone administration may increase the T/E ratio. In the last years, the determination of the carbon isotope ratio has proven to be the most promising method to help discriminate between naturally elevated T/E ratios and those reflecting T use. In this paper, an excretion study following oral administration of 40 mg testosterone undecanoate initially and 13 h later is presented. Four testosterone metabolites (androsterone, etiocholanolone, 5 alpha-androstanediol, and 5 beta-androstanediol) together with an endogenous reference (5 beta-pregnanediol) were extracted from the urines and the delta(13)C/(12)C ratio of each compound was analyzed by gas chromatography-combustion-isotope ratio mass spectrometry. The results show similar maximum delta(13)C-value variations (parts per thousand difference of delta(13)C/(12)C ratio from the isotope ratio standard) for the T metabolites and concomitant changes of the T/E ratios after administration of the first and the second dose of T. Whereas the T/E ratios as well as the androsterone, etiocholanolone and 5 alpha-androstanediol delta(13)C-values returned to the baseline 15 h after the second T administration, a decrease of the 5 beta-androstanediol delta-values could be detected for over 40 h. This suggests that measurements of 5 beta-androstanediol delta-values allow the detection of a testosterone ingestion over a longer post-administration period than other T metabolites delta(13)C-values or than the usual T/E ratio approach.
Keywords
Administration, Oral, Adult, Algorithms, Androgens, Biotransformation, Carbon Dioxide, Gas Chromatography-Mass Spectrometry, Humans, Male, Mass Spectrometry, Pregnanediol, Solvents, Testosterone
Pubmed
Web of science
Open Access
Yes
Create date
08/02/2008 18:44
Last modification date
25/09/2019 7:09
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