A comprehensive analysis of gene expression profiles in distal parts of the mouse renal tubule.

Details

Ressource 1Download: BIB_5704D6E702AC.P001.pdf (922.44 [Ko])
State: Public
Version: Author's accepted manuscript
Serval ID
serval:BIB_5704D6E702AC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A comprehensive analysis of gene expression profiles in distal parts of the mouse renal tubule.
Journal
Pflügers Archiv
Author(s)
Pradervand S., Zuber Mercier A., Centeno G., Bonny O., Firsov D.
ISSN
1432-2013[electronic], 0031-6768[linking]
Publication state
Published
Issued date
2010
Volume
460
Number
6
Pages
925-952
Language
english
Abstract
The distal parts of the renal tubule play a critical role in maintaining homeostasis of extracellular fluids. In this review, we present an in-depth analysis of microarray-based gene expression profiles available for microdissected mouse distal nephron segments, i.e., the distal convoluted tubule (DCT) and the connecting tubule (CNT), and for the cortical portion of the collecting duct (CCD; Zuber et al., Proc Natl Acad Sci USA 106:16523-16528, 2009). Classification of expressed transcripts in 14 major functional gene categories demonstrated that all principal proteins involved in maintaining the salt and water balance are represented by highly abundant transcripts. However, a significant number of transcripts belonging, for instance, to categories of G-protein-coupled receptors or serine/threonine kinases exhibit high expression levels but remain unassigned to a specific renal function. We also established a list of genes differentially expressed between the DCT/CNT and the CCD. This list is enriched by genes related to segment-specific transport functions and by transcription factors directing the development of the distal nephron or collecting ducts. Collectively, this in silico analysis provides comprehensive information about relative abundance and tissue specificity of the DCT/CNT and the CCD expressed transcripts and identifies new candidate genes for renal homeostasis.
Keywords
Kidney, Homeostasis, Membrane transport, Transport, Urinary excretion, cortical collecting duct, protein-coupled receptors, epithelial sodium-channel, nephrogenic diabetes-insipidus, clathrin-coated vesicles, na+ channel, essential-hypertension, surface expression, potassium channel, chloride channel
Pubmed
Web of science
Create date
10/11/2010 17:21
Last modification date
20/08/2019 15:11
Usage data