Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa.
Details
Serval ID
serval:BIB_56CA4BFD5F72
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa.
Journal
Clinical microbiology and infection
Working group(s)
Pyopneumagen Group
ISSN
1198-743X (Print)
ISSN-L
1198-743X
Publication state
Published
Issued date
04/2008
Peer-reviewed
Oui
Volume
14
Number
4
Pages
337-343
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Respiratory isolates of Pseudomonas aeruginosa were collected from 58 critically-ill patients with ventilator-associated pneumonia. Expression of elastase and pyocyanin was assessed semi-quantitatively, while quorum-sensing activity was assessed by quantifying the levels of the autoinducers N-3-oxododecanoyl-L-homoserine lactone (C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL). Correlations were sought between quorum-sensing activity and the expression of these two virulence factors, and all results were compared to those obtained with the laboratory reference strains PA103, a strain defective in quorum-sensing, and PAO1, a functional quorum-sensing strain. More than two-thirds of clinically pathogenic isolates had increased levels of elastase and/or pyocyanin, and high quorum-sensing activity, as assessed by autoinducer levels. However, a strong correlation between quorum-sensing activity and virulence factor production was revealed only for elastase and not for pyocyanin (C12-HSL/elastase, r = 0.7, p 2 x 10(-9); C4-HSL/elastase, r = 0.7, p 2 x 10(-9)). These data suggest that the pathogenicity of P. aeruginosa isolates from critically-ill patients with ventilator-associated pneumonia is caused, at least in part, by an increase in elastase production regulated by quorum-sensing, while increased pyocyanin production in these isolates may be regulated predominantly by mechanisms other than quorum-sensing.
Keywords
4-Butyrolactone/analogs & derivatives, 4-Butyrolactone/metabolism, Bacterial Proteins, Gene Expression Regulation, Bacterial, Humans, Pancreatic Elastase/genetics, Pancreatic Elastase/metabolism, Pneumonia, Ventilator-Associated/microbiology, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/growth & development, Pseudomonas aeruginosa/isolation & purification, Pseudomonas aeruginosa/pathogenicity, Pyocyanine/genetics, Pyocyanine/metabolism, Quorum Sensing, Virulence Factors/genetics, Virulence Factors/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
29/04/2021 9:59
Last modification date
17/07/2023 14:12