Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_56CA4BFD5F72
Type
Article: article from journal or magazin.
Collection
Publications
Title
Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa.
Journal
Clinical microbiology and infection
Author(s)
Le Berre R., Nguyen S., Nowak E., Kipnis E., Pierre M., Ader F., Courcol R., Guery B.P., Faure K.
Working group(s)
Pyopneumagen Group
ISSN
1198-743X (Print)
ISSN-L
1198-743X
Publication state
Published
Issued date
04/2008
Peer-reviewed
Oui
Volume
14
Number
4
Pages
337-343
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Respiratory isolates of Pseudomonas aeruginosa were collected from 58 critically-ill patients with ventilator-associated pneumonia. Expression of elastase and pyocyanin was assessed semi-quantitatively, while quorum-sensing activity was assessed by quantifying the levels of the autoinducers N-3-oxododecanoyl-L-homoserine lactone (C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL). Correlations were sought between quorum-sensing activity and the expression of these two virulence factors, and all results were compared to those obtained with the laboratory reference strains PA103, a strain defective in quorum-sensing, and PAO1, a functional quorum-sensing strain. More than two-thirds of clinically pathogenic isolates had increased levels of elastase and/or pyocyanin, and high quorum-sensing activity, as assessed by autoinducer levels. However, a strong correlation between quorum-sensing activity and virulence factor production was revealed only for elastase and not for pyocyanin (C12-HSL/elastase, r = 0.7, p 2 x 10(-9); C4-HSL/elastase, r = 0.7, p 2 x 10(-9)). These data suggest that the pathogenicity of P. aeruginosa isolates from critically-ill patients with ventilator-associated pneumonia is caused, at least in part, by an increase in elastase production regulated by quorum-sensing, while increased pyocyanin production in these isolates may be regulated predominantly by mechanisms other than quorum-sensing.
Keywords
4-Butyrolactone/analogs & derivatives, 4-Butyrolactone/metabolism, Bacterial Proteins, Gene Expression Regulation, Bacterial, Humans, Pancreatic Elastase/genetics, Pancreatic Elastase/metabolism, Pneumonia, Ventilator-Associated/microbiology, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/growth & development, Pseudomonas aeruginosa/isolation & purification, Pseudomonas aeruginosa/pathogenicity, Pyocyanine/genetics, Pyocyanine/metabolism, Quorum Sensing, Virulence Factors/genetics, Virulence Factors/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
29/04/2021 9:59
Last modification date
17/07/2023 14:12
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