Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa

Details

Serval ID
serval:BIB_56CA4BFD5F72
Type
Article: article from journal or magazin.
Collection
Publications
Title
Quorum-sensing activity and related virulence factor expression in clinically pathogenic isolates of Pseudomonas aeruginosa
Journal
Clin Microbiol Infect
Author(s)
Le Berre R., Nguyen S., Nowak E., Kipnis E., Pierre M., Ader F., Courcol R., Guery B. P., Faure K.
Working group(s)
Pyopneumagen, Group
ISSN
1198-743X (Print)
ISSN-L
1198-743X
Publication state
Published
Issued date
04/2008
Volume
14
Number
4
Pages
337-43
Language
english
Notes
Le Berre, R
Nguyen, S
Nowak, E
Kipnis, E
Pierre, M
Ader, F
Courcol, R
Guery, B P
Faure, K
eng
Research Support, Non-U.S. Gov't
England
Clin Microbiol Infect. 2008 Apr;14(4):337-43. doi: 10.1111/j.1469-0691.2007.01925.x. Epub 2008 Jan 7.
Abstract
Respiratory isolates of Pseudomonas aeruginosa were collected from 58 critically-ill patients with ventilator-associated pneumonia. Expression of elastase and pyocyanin was assessed semi-quantitatively, while quorum-sensing activity was assessed by quantifying the levels of the autoinducers N-3-oxododecanoyl-L-homoserine lactone (C12-HSL) and N-butanoyl-L-homoserine lactone (C4-HSL). Correlations were sought between quorum-sensing activity and the expression of these two virulence factors, and all results were compared to those obtained with the laboratory reference strains PA103, a strain defective in quorum-sensing, and PAO1, a functional quorum-sensing strain. More than two-thirds of clinically pathogenic isolates had increased levels of elastase and/or pyocyanin, and high quorum-sensing activity, as assessed by autoinducer levels. However, a strong correlation between quorum-sensing activity and virulence factor production was revealed only for elastase and not for pyocyanin (C12-HSL/elastase, r = 0.7, p 2 x 10(-9); C4-HSL/elastase, r = 0.7, p 2 x 10(-9)). These data suggest that the pathogenicity of P. aeruginosa isolates from critically-ill patients with ventilator-associated pneumonia is caused, at least in part, by an increase in elastase production regulated by quorum-sensing, while increased pyocyanin production in these isolates may be regulated predominantly by mechanisms other than quorum-sensing.
Keywords
4-Butyrolactone/analogs & derivatives/metabolism, Bacterial Proteins, *Gene Expression Regulation, Bacterial, Humans, Pancreatic Elastase/genetics/metabolism, Pneumonia, Ventilator-Associated/*microbiology, Pseudomonas Infections/*microbiology, Pseudomonas aeruginosa/growth & development/isolation &, purification/*pathogenicity, Pyocyanine/genetics/metabolism, *Quorum Sensing, Virulence Factors/genetics/*metabolism
Pubmed
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29/04/2021 10:59
Last modification date
30/04/2021 6:38
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