Ablation of glucokinase-expressing tanycytes impacts energy balance and increases adiposity in mice.

Details

Serval ID
serval:BIB_569B8719E376
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ablation of glucokinase-expressing tanycytes impacts energy balance and increases adiposity in mice.
Journal
Molecular metabolism
Author(s)
Rohrbach A., Caron E., Dali R., Brunner M., Pasquettaz R., Kolotuev I., Santoni F., Thorens B., Langlet F.
ISSN
2212-8778 (Electronic)
ISSN-L
2212-8778
Publication state
In Press
Peer-reviewed
Oui
Volume
53
Pages
101311
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Glucokinase (GCK) is critical for glucosensing. In rats, GCK is expressed in hypothalamic tanycytes and appears to play an essential role in feeding behavior. In this study, we investigated the distribution of GCK-expressing tanycytes in mice and their role in the regulation of energy balance.
In situ hybridization, reporter gene assay, and immunohistochemistry were used to assess GCK expression along the third ventricle in mice. To evaluate the impact of GCK-expressing tanycytes on arcuate neuron function and mouse physiology, Gck deletion along the ventricle was achieved using loxP/Cre recombinase technology in adult mice.
GCK expression was low along the third ventricle, but detectable in tanycytes facing the ventromedial arcuate nucleus from bregma -1.5 to -2.2. Gck deletion induced the death of this tanycyte subgroup through the activation of the BAD signaling pathway. The ablation of GCK-expressing tanycytes affected different aspects of energy balance, leading to an increase in adiposity in mice. This phenotype was systematically associated with a defect in NPY neuron function. In contrast, the regulation of glucose homeostasis was mostly preserved, except for glucoprivic responses.
This study describes the role of GCK in tanycyte biology and highlights the impact of tanycyte loss on the regulation of energy balance.
Keywords
Apoptosis, Energy Balance, Glucokinase, Glucose homeostasis, Hypothalamus, Tanycytes, Energy balance
Pubmed
Web of science
Open Access
Yes
Create date
06/08/2021 12:20
Last modification date
16/10/2021 6:34
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