Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density.

Details

Serval ID
serval:BIB_5686BF1354AC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density.
Journal
European journal of immunology
Author(s)
Gilfillan C.B., Wang C., Mohsen M.O., Rufer N., Hebeisen M., Allard M., Verdeil G., Irvine D.J., Bachmann M.F., Speiser D.E.
ISSN
1521-4141 (Electronic)
ISSN-L
0014-2980
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
50
Number
4
Pages
505-514
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
It is known that for achieving high affinity antibody responses, vaccines must be optimized for antigen dose/density, and the prime/boost interval should be at least 4 weeks. Similar knowledge is lacking for generating high avidity T-cell responses. The functional avidity (FA) of T cells, describing responsiveness to peptide, is associated with the quality of effector function and the protective capacity in vivo. Despite its importance, the FA is rarely determined in T-cell vaccination studies. We addressed the question whether different time intervals for short-term homologous vaccinations impact the FA of CD8 T-cell responses. Four-week instead of 2-week intervals between priming and boosting with potent subunit vaccines in C57BL/6 mice did not improve FA. Equally, similar FA was observed after vaccination with virus-like particles displaying low versus high antigen densities. Interestingly, FA was stable in vivo but not in vitro, depending on the antigen dose and the time interval since T-cell activation, as observed in murine monoclonal T cells. Our findings suggest dynamic in vivo modulation for equal FA. We conclude that low antigen density vaccines or a minimal 4-week prime/boost interval are not crucial for the T-cell's FA, in contrast to antibody responses.
Keywords
Animals, Antibody Formation, Antigen Presentation, Antigens/immunology, Antigens/metabolism, CD8-Positive T-Lymphocytes/immunology, Cells, Cultured, Immunization, Secondary, Mice, Mice, Inbred C57BL, Peptides/immunology, Peptides/metabolism, Protein Binding, Receptors, Antigen, T-Cell/metabolism, Vaccination, Vaccines, Subunit/immunology, Vaccines, Virus-Like Particle/immunology, Avidity regulation, Functional avidity, Prime/boost, T-cell receptor affinity, T-cell vaccination
Pubmed
Web of science
Open Access
Yes
Create date
04/12/2019 23:22
Last modification date
18/08/2020 6:21
Usage data