Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density.
Details
Serval ID
serval:BIB_5686BF1354AC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density.
Journal
European journal of immunology
ISSN
1521-4141 (Electronic)
ISSN-L
0014-2980
Publication state
Published
Issued date
04/2020
Peer-reviewed
Oui
Volume
50
Number
4
Pages
505-514
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
It is known that for achieving high affinity antibody responses, vaccines must be optimized for antigen dose/density, and the prime/boost interval should be at least 4 weeks. Similar knowledge is lacking for generating high avidity T-cell responses. The functional avidity (FA) of T cells, describing responsiveness to peptide, is associated with the quality of effector function and the protective capacity in vivo. Despite its importance, the FA is rarely determined in T-cell vaccination studies. We addressed the question whether different time intervals for short-term homologous vaccinations impact the FA of CD8 T-cell responses. Four-week instead of 2-week intervals between priming and boosting with potent subunit vaccines in C57BL/6 mice did not improve FA. Equally, similar FA was observed after vaccination with virus-like particles displaying low versus high antigen densities. Interestingly, FA was stable in vivo but not in vitro, depending on the antigen dose and the time interval since T-cell activation, as observed in murine monoclonal T cells. Our findings suggest dynamic in vivo modulation for equal FA. We conclude that low antigen density vaccines or a minimal 4-week prime/boost interval are not crucial for the T-cell's FA, in contrast to antibody responses.
Keywords
Animals, Antibody Formation, Antigen Presentation, Antigens/immunology, Antigens/metabolism, CD8-Positive T-Lymphocytes/immunology, Cells, Cultured, Immunization, Secondary, Mice, Mice, Inbred C57BL, Peptides/immunology, Peptides/metabolism, Protein Binding, Receptors, Antigen, T-Cell/metabolism, Vaccination, Vaccines, Subunit/immunology, Vaccines, Virus-Like Particle/immunology, Avidity regulation, Functional avidity, Prime/boost, T-cell receptor affinity, T-cell vaccination
Pubmed
Web of science
Open Access
Yes
Create date
04/12/2019 23:22
Last modification date
30/04/2021 7:10