Detection and quantification of lorazepam in human hair by GC-MS/NCI in a case of traffic accident.

Details

Serval ID
serval:BIB_564D10705C7B
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Title
Detection and quantification of lorazepam in human hair by GC-MS/NCI in a case of traffic accident.
Journal
International Journal of Legal Medicine
Author(s)
Cirimele V., Kintz P., Mangin P.
ISSN
0937-9827[print], 0937-9827[linking]
Publication state
Published
Issued date
1996
Volume
108
Number
5
Pages
265-267
Language
english
Abstract
A traffic accident caused by a man who declared that he was driving under influence of drugs (Temesta), led our laboratory to develop a procedure for the detection and the quantification of lorazepam in human hair. The method involves decontamination of hair with dichloromethane, incubation in Soerensen buffer (pH 7.6) in the presence of lorazepam-d4, liquid-liquid extraction with diethylether-chloroform (80:20, v/v) at pH 8.4, derivatization by silylation and detection by GC-MS/NCI. The increasing concentrations of lorazepam from the end to the roots of a 16-cm-long hair strand (i.e. 31 pg/mg, 40 pg/mg and 49 pg/mg) proved that the driver had taken the drug over a long period of time.
Keywords
Accidents, Traffic/legislation & jurisprudence, Anti-Anxiety Agents/administration & dosage, Anti-Anxiety Agents/adverse effects, Anxiety Disorders/drug therapy, Dose-Response Relationship, Drug, Gas Chromatography-Mass Spectrometry, Hair/chemistry, Humans, Lorazepam/administration & dosage, Lorazepam/adverse effects, Male, Middle Aged
Pubmed
Create date
19/10/2010 17:02
Last modification date
20/08/2019 15:10
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