Direct identification of recombinant vaccinia virus plaques by PCR.
Details
Serval ID
serval:BIB_5611A0D3DF0C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Direct identification of recombinant vaccinia virus plaques by PCR.
Journal
Journal of Virological Methods
ISSN
0166-0934 (Print)
ISSN-L
0166-0934
Publication state
Published
Issued date
12/1991
Volume
35
Number
2
Pages
137-141
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
A fast method for the identification of recombinant vaccinia viruses directly from individual plaques is described. Plaques are picked, resuspended in PBS-A and processed for PCR using two 'universal' primers. The amplified sequences are analyzed by agarose gel electrophoresis. This procedure allows discrimination between spontaneously arising TK-negative mutants, which do not carry the inserted gene, and the desired TK-negative recombinants resulting from insertional inactivation of the TK gene.
Keywords
Base Sequence, Cells, Cultured, Genetic Vectors, Molecular Sequence Data, Polymerase Chain Reaction, Recombination, Genetic, Vaccinia virus/genetics, Vaccinia virus/isolation & purification, Viral Plaque Assay
Pubmed
Web of science
Create date
24/01/2008 10:43
Last modification date
20/08/2019 14:10