Establishment and Characterization of a Functionally Competent Type 2 Conventional Dendritic Cell Line.

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Serval ID
serval:BIB_55E5B7D249E0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Establishment and Characterization of a Functionally Competent Type 2 Conventional Dendritic Cell Line.
Journal
Frontiers in immunology
Author(s)
Pigni M., Ashok D., Stevanin M., Acha-Orbea H.
ISSN
1664-3224 (Electronic)
ISSN-L
1664-3224
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
9
Pages
1912
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Dendritic cells (DCs) are the most potent antigen presenting cells and possess an incomparable ability to activate and instruct T cells, which makes them one of the cornerstones in the regulation of the cross-talk between innate and adaptive immunity. Therefore, a deep understanding of DC biology lays the foundations to describe and to harness the mechanisms that regulate the development of the adaptive response, with clear implications in a vast array of fields such as the study of autoimmune diseases and the development of new vaccines. However, the great difficulty to obtain large quantities of viable non-activated DCs for experimentation have considerably hindered the progress of DC research. Several strategies have been proposed to overcome these limitations by promoting an increase of DC abundance in vivo, by inducing DC development from DC progenitors in vitro and by generating stable DC lines. In the past years, we have described a method to derive immortalized stable DC lines, named MutuDCs, from the spleens of Mushi1 mice, a transgenic mouse strain that express the simian virus 40 Large T-oncogene in the DCs. The comparison of these DC lines with the vast variety of DC subsets described in vivo has shown that all the MutuDC lines that we have generated so far have phenotypic and functional features of type 1 conventional DCs (cDC1s). With the purpose of deriving DC lines with characteristics of type 2 conventional DCs (cDC2s), we bred a new Batf3 <sup>-/-</sup> Mushi1 murine line in which the development of the cDC1 subset is severely defective. The new MutuDC line that we generated from Batf3 <sup>-/-</sup> Mushi1 mice was phenotypically and functionally characterized in this work. Our results demonstrated that all the tested characteristics of this new cell line, including the expression of subset-determining transcription factors, the profile of cytokine production and the ability to present antigens, are comparable with the features of splenic CD4 <sup>-</sup> cDC2s. Therefore, we concluded that our new cell line, that we named CD4 <sup>-</sup> MutuDC2 line, represents a valuable model for the CD4 <sup>-</sup> cDC2 subset.
Keywords
Adaptive Immunity, Animals, Cell Line, Dendritic Cells/cytology, Dendritic Cells/immunology, Mice, Mice, Knockout, Spleen/cytology, Spleen/immunology, cDC1, cDC2, cell culture, cell line, conventional DC subset, dendritic cell, mouse, spleen
Pubmed
Web of science
Open Access
Yes
Create date
07/09/2018 8:27
Last modification date
28/09/2019 6:08
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