Electrophoretic characteristics of monoclonal immunoglobulin G of different subclasses.

Details

Serval ID
serval:BIB_55AC7E50F8EA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Electrophoretic characteristics of monoclonal immunoglobulin G of different subclasses.
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
Author(s)
Vu D.H., Schneider P., Tissot J.D.
ISSN
1570-0232
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
771
Number
1-2
Pages
355-68
Language
english
Notes
Publication types: Journal Article - Publication Status: ppublish
Abstract
Monoclonal IgG are commonly observed in various B cell disorders, of which multiple myeloma is the most clinically relevant. In a series of serum samples, we identified by immunofixation 73 monoclonal IgG, including 63 IgG(1), 4 IgG(2), 5 IgG(3), and 1 IgG(4). The light chains were of kappa type in 45 cases, and of lambda type in 28 cases. These monoclonal IgG were further characterized by high resolution two-dimensional polyacrylamide gel electrophoresis (2-DE) in various isoelectric focusing conditions, as well as by 3-DE (2-DE of the proteins extracted from agarose after serum protein agarose electrophoresis). After 2-DE, 38 out of 73 monoclonal gamma chains (52%) were visualized using immobilized pH 3-10 gradients for isoelectric focusing. In 6 cases (8%), gamma chains were only detected using alkaline immobilized pH 6-11 gradients. In 3 cases (4%), 3-DE revealed monoclonal gamma chains hidden by polyclonal gamma chains. Finally, in 26 cases (36%), no monoclonal gamma chains were clearly visualized. Sixty-one monoclonal light chains (84%) were detected using immobilized pH 3-10 gradients, whereas 12 (16%) were not. Monoclonal gamma chains and light chains were highly heterogeneous in terms of pI and M(r). However, a statistically significant correlation (P<0.05) was observed between the position of the monoclonal IgG in agarose gel and the pI of their heavy and light chains (R=0.733, multiple linear regression). Because of the extreme diversity of their heavy and light chains, it appears that a classification of monoclonal IgG based only on their electrophoretic properties is not possible.
Keywords
Electrophoresis, Gel, Two-Dimensional, Humans, Immunoelectrophoresis, Immunoglobulin G
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 14:10
Usage data