Synthetic peptides as antigens and competitors in recognition by H-2-restricted cytolytic T cells specific for HLA

Details

Serval ID
serval:BIB_559E2E96798C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Synthetic peptides as antigens and competitors in recognition by H-2-restricted cytolytic T cells specific for HLA
Journal
Journal of Experimental Medicine
Author(s)
Maryanski  J. L., Pala  P., Cerottini  J. C., Corradin  G.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
04/1988
Volume
167
Number
4
Pages
1391-405
Notes
Comparative Study
Journal Article --- Old month value: Apr 1
Abstract
The specificity of peptide recognition by a number of Kd-restricted CTL clones specific for HLA-CW3 or HLA-A24 was investigated. The CTL clones were derived from DBA/2 (H-2d) mice immunized with syngeneic P815 mouse cells transfected with genes encoding HLA-CW3 or HLA-A24 class I molecules. We had previously shown that CTL clones that lysed P815-CW3 transfectant target cells could lyse P815 (HLA-) target cells incubated with synthetic CW3 peptides corresponding to the COOH-terminal end of the alpha 2 domain. In the present study, we found that Kd-restricted CTL clones that lysed P815-A24 transfectant target cells recognized a synthetic peptide from the same region (residues 170-182) of the A24 molecule. CW3 and A24 differ by only one amino acid within this region. Recognition of CW3 or A24 peptides corresponded exactly with lysis of P815-HLA transfectants both for clones that mutually exclusively lysed CW3 or A24 transfectant target cells and for CW3/A24 crossreactive CTL clones. The latter CTL clones that lysed both CW3 and A24 transfectant target cells showed a clear preference for the peptide corresponding to the immunizing HLA allele. The homologous CW3 and A24 peptides could compete with each other for recognition, in contrast to a peptide from the same region of HLA-B7. Peptides from the corresponding region of the endogenous Kd and Dd/Ld molecules could also inhibit recognition of CW3 and A24 peptides. Competition with peptides apparently occurred at the level of the target cell. These results are consistent with a model whereby MHC class I molecules position protein fragments or peptides for specific recognition by T cells.
Keywords
Amino Acid Sequence Animals H-2 Antigens/*immunology HLA Antigens/*immunology Humans Mast-Cell Sarcoma/pathology Mice Mice, Inbred DBA/immunology Molecular Sequence Data Peptide Fragments/chemical synthesis/*immunology T-Lymphocytes, Cytotoxic/*immunology Tumor Cells, Cultured/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:14
Last modification date
20/08/2019 14:10
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