Allergen-derived long peptide immunotherapy down-regulates specific IgE response and protects from anaphylaxis

Details

Serval ID
serval:BIB_556A085642B9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Allergen-derived long peptide immunotherapy down-regulates specific IgE response and protects from anaphylaxis
Journal
European Journal of Immunology
Author(s)
von Garnier  C., Astori  M., Kettner  A., Dufour  N., Heusser  C., Corradin  G., Spertini  F.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
06/2000
Volume
30
Number
6
Pages
1638-45
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
To evaluate a long peptide-based allergy vaccine in a murine model, CBA/J mice were sensitized with low dose alum-adsorbed phospholipase A2 (PLA2), a major bee venom allergen. Presensitized mice were treated by daily i.p. injections of a mixture of three long overlapping peptides (44- to 60-mer) spanning the entire PLA2 molecule (100 microg/peptide) for 6 consecutive days. This therapeutic approach induced a sharp drop in PLA2-specific IgE, an increase in specific IgG2a, and a marked T cell hyporesponsiveness. T cell cytokine secretion was characterized by a shift from a Th2 to a Th1 profile. Prophylactic treatment of naive mice with long peptides prior to sensitization with PLA2 induced a comparable modulation of B and T cell responses. Upon i.p. challenge with native PLA2, presensitized mice treated with the long peptide mixture were fully protected from anaphylaxis. This indicated that allergen-derived long overlapping peptides were safe and able to modulate an established Th2 response or to prevent its development. Furthermore, long peptide-based immunotherapy provided clinical protection against anaphylaxis, thus appearing as a promising approach of the therapy of allergic diseases.
Keywords
Allergens/administration & dosage/*immunology Anaphylaxis/*prevention & control Animals Bee Venoms/administration & dosage/chemical synthesis/*immunology Cell Division Down-Regulation/*immunology Female Immunoglobulin E/*blood Immunoglobulin G/blood Immunotherapy Injections, Intraperitoneal Mice Mice, Inbred CBA Peptides/administration & dosage/chemical synthesis/immunology Phospholipases A/administration & dosage/chemical synthesis/*immunology T-Lymphocytes/immunology Th1 Cells/immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:55
Last modification date
20/08/2019 14:10
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