tRNA 2'-O-methylation by a duo of TRM7/FTSJ1 proteins modulates small RNA silencing in Drosophila.

Details

Serval ID
serval:BIB_54F1E8540412
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
tRNA 2'-O-methylation by a duo of TRM7/FTSJ1 proteins modulates small RNA silencing in Drosophila.
Journal
Nucleic acids research
Author(s)
Angelova M.T., Dimitrova D.G., Da Silva B., Marchand V., Jacquier C., Achour C., Brazane M., Goyenvalle C., Bourguignon-Igel V., Shehzada S., Khouider S., Lence T., Guerineau V., Roignant J.Y., Antoniewski C., Teysset L., Bregeon D., Motorin Y., Schaefer M.R., Carré C.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
28/02/2020
Peer-reviewed
Oui
Volume
48
Number
4
Pages
2050-2072
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
2'-O-Methylation (Nm) represents one of the most common RNA modifications. Nm affects RNA structure and function with crucial roles in various RNA-mediated processes ranging from RNA silencing, translation, self versus non-self recognition to viral defense mechanisms. Here, we identify two Nm methyltransferases (Nm-MTases) in Drosophila melanogaster (CG7009 and CG5220) as functional orthologs of yeast TRM7 and human FTSJ1. Genetic knockout studies together with MALDI-TOF mass spectrometry and RiboMethSeq mapping revealed that CG7009 is responsible for methylating the wobble position in tRNAPhe, tRNATrp and tRNALeu, while CG5220 methylates position C32 in the same tRNAs and also targets additional tRNAs. CG7009 or CG5220 mutant animals were viable and fertile but exhibited various phenotypes such as lifespan reduction, small RNA pathways dysfunction and increased sensitivity to RNA virus infections. Our results provide the first detailed characterization of two TRM7 family members in Drosophila and uncover a molecular link between enzymes catalyzing Nm at specific tRNAs and small RNA-induced gene silencing pathways.
Keywords
Animals, Drosophila melanogaster/genetics, Gene Expression Regulation/genetics, Gene Silencing, Humans, Methylation, Methyltransferases/genetics, Nuclear Proteins/genetics, RNA Interference, RNA, Transfer/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, tRNA Methyltransferases/genetics
Pubmed
Open Access
Yes
Create date
20/01/2020 14:01
Last modification date
03/04/2020 6:19
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