Enhancement of human adipose-derived stem cell expansion and stability for clinical use

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Serval ID
serval:BIB_54D80FFE0684
Type
PhD thesis: a PhD thesis.
Collection
Publications
Institution
Title
Enhancement of human adipose-derived stem cell expansion and stability for clinical use
Author(s)
Krähenbühl S. M.
Director(s)
LAURENT-APPLEGATE L. A., RAFFOUL W.
Institution details
Université de Lausanne, Faculté de biologie et médecine
Address
Faculté de biologie et de médecine Université de Lausanne CH-1015 Lausanne SUISSE
Publication state
Accepted
Issued date
2016
Language
english
Number of pages
8
Abstract
Co-culture techniques associating both dermal fibroblasts and epidermal keratinocytes have shown to have better clinical outcome
than keratinocyte culture alone for the treatment of severe burns.
Since fat grafting has been shown to improve scar remodelling, new techniques such as cell-therapy-assisted surgical reconstruction
with isolated and expanded autologous adipose-derived stem cells (ASCs) would be of benefit to increase graft acceptation. Therefore,
integrating ASCs into standardized procedures for cultured skin grafting could be of benefit for the patient if cell quality and quantity could be maintained. The purpose of this study was to evaluate ASC processing from adult tissue with simple isolation (without enzymatic steps), expansion (low density of 325-3,000 cells/cm2) and storage conditions to assure methods to enhance the cellular resistance when transferred back to the patient. Co-culture with cell-banked skin progenitor cells (FE002-SK2) showed an increase of 40-50% ASCs yield at high passages alongside with a better
preservation of morphology, proper adipogenic and osteogenic differentiation and efficient biocompatibility with 3D collagen
scaffolds. ASCs can be considered as a valuable additional cell source to be delivered in biological bandages to the patient in a
need of tissue reconstruction such as burn patients.
Keywords
Adipose-derived stem cells, Cell stability, Co-culture, Fat grafting, Lipofilling, Burn treatment, Biological bandages
Create date
02/05/2016 8:56
Last modification date
20/08/2019 15:09
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