Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_54CB5D32893E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport.
Journal
Life science alliance
Author(s)
Rickman O.J., Guignard E., Chabanon T., Bertoldi G., Auberson M., Hummler E.
ISSN
2575-1077 (Electronic)
ISSN-L
2575-1077
Publication state
Published
Issued date
03/2024
Peer-reviewed
Oui
Volume
7
Number
3
Pages
e202302304
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
The mouse cortical collecting duct cell line presents a tight epithelium with regulated ion and water transport. The epithelial sodium channel (ENaC) is localized in the apical membrane and constitutes the rate-limiting step for sodium entry, thereby enabling transepithelial transport of sodium ions. The membrane-bound serine protease Tmprss2 is co-expressed with the alpha subunit of ENaC. αENaC gene expression followed the Tmprss2 expression, and the absence of Tmprss2 resulted not only in down-regulation of αENaC gene and protein expression but also in abolished transepithelial sodium transport. In addition, RNA-sequencing analyses unveiled drastic down-regulation of the membrane-bound protease CAP3/St14, the epithelial adhesion molecule EpCAM, and the tight junction proteins claudin-7 and claudin-3 as also confirmed by immunohistochemistry. In summary, our data clearly demonstrate a dual role of Tmprss2 in maintaining not only ENaC-mediated transepithelial but also EpCAM/claudin-7-mediated paracellular barrier; the tight epithelium of the mouse renal mCCD cells becomes leaky. Our working model proposes that Tmprss2 acts via CAP3/St14 on EpCAM/claudin-7 tight junction complexes and through regulating transcription of αENaC on ENaC-mediated sodium transport.
Keywords
Animals, Mice, Biological Transport/physiology, Claudins/genetics, Claudins/metabolism, Epithelial Cell Adhesion Molecule/metabolism, Ion Transport, Sodium/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2024 15:25
Last modification date
09/08/2024 15:59
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