Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport.
Details
Serval ID
serval:BIB_54CB5D32893E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tmprss2 maintains epithelial barrier integrity and transepithelial sodium transport.
Journal
Life science alliance
ISSN
2575-1077 (Electronic)
ISSN-L
2575-1077
Publication state
Published
Issued date
03/2024
Peer-reviewed
Oui
Volume
7
Number
3
Pages
e202302304
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
The mouse cortical collecting duct cell line presents a tight epithelium with regulated ion and water transport. The epithelial sodium channel (ENaC) is localized in the apical membrane and constitutes the rate-limiting step for sodium entry, thereby enabling transepithelial transport of sodium ions. The membrane-bound serine protease Tmprss2 is co-expressed with the alpha subunit of ENaC. αENaC gene expression followed the Tmprss2 expression, and the absence of Tmprss2 resulted not only in down-regulation of αENaC gene and protein expression but also in abolished transepithelial sodium transport. In addition, RNA-sequencing analyses unveiled drastic down-regulation of the membrane-bound protease CAP3/St14, the epithelial adhesion molecule EpCAM, and the tight junction proteins claudin-7 and claudin-3 as also confirmed by immunohistochemistry. In summary, our data clearly demonstrate a dual role of Tmprss2 in maintaining not only ENaC-mediated transepithelial but also EpCAM/claudin-7-mediated paracellular barrier; the tight epithelium of the mouse renal mCCD cells becomes leaky. Our working model proposes that Tmprss2 acts via CAP3/St14 on EpCAM/claudin-7 tight junction complexes and through regulating transcription of αENaC on ENaC-mediated sodium transport.
Keywords
Animals, Mice, Biological Transport/physiology, Claudins/genetics, Claudins/metabolism, Epithelial Cell Adhesion Molecule/metabolism, Ion Transport, Sodium/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2024 15:25
Last modification date
09/08/2024 15:59