Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa.

Details

Serval ID
serval:BIB_54BD1C32D6A7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa.
Journal
Communications biology
Author(s)
Testa S., Berger S., Piccardi P., Oechslin F., Resch G., Mitri S.
ISSN
2399-3642 (Electronic)
ISSN-L
2399-3642
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
2
Pages
405
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Bacterial viruses, or phage, are key members of natural microbial communities. Yet much research on bacterial-phage interactions has been conducted in liquid cultures involving single bacterial strains. Here we explored how bacterial diversity affects the success of lytic phage in structured communities. We infected a sensitive Pseudomonas aeruginosa strain PAO1 with a lytic phage Pseudomonas 352 in the presence versus absence of an insensitive P. aeruginosa strain PA14, in liquid culture versus colonies on agar. We found that both in liquid and in colonies, inter-strain competition reduced resistance evolution in the susceptible strain and decreased phage population size. However, while all sensitive bacteria died in liquid, bacteria in colonies could remain sensitive yet escape phage infection, due mainly to reduced growth in colony centers. In sum, spatial structure can protect bacteria against phage infection, while the presence of competing strains reduces the evolution of resistance to phage.
Keywords
Bacteriophages, Biofilms, Microbial ecology
Pubmed
Web of science
Open Access
Yes
Create date
15/11/2019 21:48
Last modification date
10/12/2019 7:17
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