[Contribution of bacterial detachment by enzymatic or physical methods to the diagnosis of foreign material infections and chronic osteomyelitis]

Details

Serval ID
serval:BIB_547DA0021168
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
[Contribution of bacterial detachment by enzymatic or physical methods to the diagnosis of foreign material infections and chronic osteomyelitis]
Journal
Pathologie-Biologie
Author(s)
Etesse-Carsenti  H., Masseyeff  M. F., Entenza  J., Monnot  F., Giaume  F., Barbarin  A., Rosset  M. J., Argenson  C., Dellamonica  P.
ISSN
0369-8114 (Print)
Publication state
Published
Issued date
01/1992
Volume
40
Number
1
Pages
40-6
Notes
Comparative Study
English Abstract
In Vitro
Journal Article --- Old month value: Jan
Abstract
Bacterial adhesion is a crucial factor in the pathogenicity of numerous bacterial species. This property plays such a great role in infections of foreign material that removal of the material is the only effective therapy. The electron microscopy studies performed by Christensen have demonstrated that infected foreign material harbors bacteria which are often coated with slime and therefore escape detection by conventional microbiological methods. in view of these properties, enzymatic or physical detachment of adherent bacteria was attempted. 231 specimens from a variety of materials (catheters, heart valves, drains, bone biopsies) which yielded negative results after 15 days culture in Schaedler medium were either exposed to trypsin or sonicated. Among catheter specimens, 44.7% yielded positive bacteriologic results; pathogens recovered included 8.3% micrococci, 5.1% nongroupable streptococci, 1.7% group D streptococci, 2.9% Enterobacteria, and 23.3% coagulase-negative staphylococci. Among the bone specimens, the improvement in yield was 64.7%; except in one case, the pathogens recovered were consistent with previously initiated treatments. This bacterial detachment method should be studied in a larger number of specimens to determine its diagnostic value as compared with conventional techniques.
Keywords
Bacterial Adhesion/*drug effects Bioprosthesis Catheters, Indwelling/adverse effects Chronic Disease Gram-Positive Bacterial Infections/diagnosis/microbiology Heart Valve Prosthesis/adverse effects Humans Osteomyelitis/*diagnosis/etiology/microbiology Retrospective Studies Sonication/*diagnostic use Staphylococcal Infections/*diagnosis/etiology/microbiology Staphylococcus epidermidis/isolation & purification Trypsin/*pharmacology
Pubmed
Web of science
Create date
24/01/2008 14:45
Last modification date
20/08/2019 15:09
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