Cloning of a carcinoembryonic antigen gene family member expressed in leukocytes of chronic myeloid leukemia patients and bone marrow
Details
Serval ID
serval:BIB_5437E08CA02D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cloning of a carcinoembryonic antigen gene family member expressed in leukocytes of chronic myeloid leukemia patients and bone marrow
Journal
Cancer Research
ISSN
0008-5472 (Print)
Publication state
Published
Issued date
10/1990
Volume
50
Number
20
Pages
6534-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Research Support, Non-U.S. Gov't --- Old month value: Oct 15
Abstract
The carcinoembryonic antigen (CEA) gene family belongs to the immunoglobulin superfamily and can be subdivided into the CEA and pregnancy-specific glycoprotein subgroups. The basic structure of the encoded proteins consists of, in addition to a leader, one IgV-like and 2, 3, or 6 IgC-like domains. These domains are followed by varying COOH-terminal regions responsible for secretion, transmembrane anchoring, or insertion into the membrane by a glycosyl phosphatidylinositol tail. Here we report on the characterization of CGM6, a new member of the CEA gene subgroup, by complementary DNA cloning. The deduced coding region comprises 349 amino acids and consists of a leader, one IgV-like, two IgC-like domains, and a hydrophobic region, which is replaced by a glycosyl phosphatidylinositol moiety in the mature protein. CGM6 transcripts were only found thus far in leukocytes of chronic myeloid leukemia patients, in normal bone marrow, and in marginal amounts in normal granulocytes. The CGM6 gene product might, therefore, represent a myeloid marker. Analyses of CGM6 protein-expressing HeLa transfectants with monoclonal antibodies strongly indicate that the CGM6 gene codes for the CEA family member NCA-95.
Keywords
*Antigens, Neoplasm
Base Sequence
Bone Marrow/*immunology
Carcinoembryonic Antigen/*genetics
*Cell Adhesion Molecules
*Cloning, Molecular
DNA/analysis
Glycoproteins/genetics
Humans
Leukemia, Myeloid, Chronic/*genetics
Leukocytes/*immunology
Molecular Sequence Data
RNA, Messenger/analysis
Pubmed
Web of science
Create date
25/01/2008 11:27
Last modification date
20/08/2019 14:09