Injection of partially purified estrogen receptor protein from Xenopus liver nuclei into oocytes activates the silent vitellogenin locus.

Details

Serval ID
serval:BIB_5423DC52E7FC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Injection of partially purified estrogen receptor protein from Xenopus liver nuclei into oocytes activates the silent vitellogenin locus.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Knowland J., Theulaz I., Wright C.V., Wahli W.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
09/1984
Volume
81
Number
18
Pages
5777-5781
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Injection of extracts from Xenopus liver nuclei that are enriched 2000 times in estradiol receptor into Xenopus oocytes induces transcription of the silent vitellogenin locus, which is activated in liver by estradiol, but not of the albumin locus, which is active in liver but suppressed by high levels of estradiol. Transcription initiates within the 5'-end region of the gene we have studied and probably continues into the 3' third. The activation seems to be very efficient, but most of the primary transcripts are probably rapidly and inaccurately processed. New proteins are also made and secreted by the oocytes.
Keywords
Animals, Base Sequence, Cell Nucleus/physiology, Estradiol/physiology, Female, Genes, Lipoproteins/genetics, Liver/physiology, Nucleic Acid Hybridization, Oocytes/physiology, RNA, Messenger/genetics, Receptors, Estradiol, Serum Albumin/genetics, Transcription, Genetic, Vitellogenins/genetics, Vitellogenins/isolation & purification, Xenopus
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:04
Last modification date
20/08/2019 14:09
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