Antibodies That Block or Activate Mouse B Cell Activating Factor of the Tumor Necrosis Factor (TNF) Family (BAFF), Respectively, Induce B Cell Depletion or B Cell Hyperplasia.
Details
State: Public
Version: author
Serval ID
serval:BIB_5303DB297B39
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Antibodies That Block or Activate Mouse B Cell Activating Factor of the Tumor Necrosis Factor (TNF) Family (BAFF), Respectively, Induce B Cell Depletion or B Cell Hyperplasia.
Journal
The Journal of biological chemistry
ISSN
1083-351X (Electronic)
ISSN-L
0021-9258
Publication state
Published
Issued date
16/09/2016
Peer-reviewed
Oui
Volume
291
Number
38
Pages
19826-19834
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
B cell activating factor of the TNF family (BAFF), also known as B lymphocyte stimulator, is a ligand required for the generation and maintenance of B lymphocytes. In this study, the ability of different monoclonal antibodies to recognize, inhibit, or activate mouse BAFF was investigated. One of them, a mouse IgG1 named Sandy-2, prevented the binding of BAFF to all of its receptors, BAFF receptor, transmembrane activator and calcium modulating ligand interactor, and B cell maturation antigen, at a stoichiometric ratio; blocked the activity of mouse BAFF on a variety of cell-based reporter assays; and antagonized the prosurvival action of BAFF on primary mouse B cells in vitro A single administration of Sandy-2 in mice induced B cell depletion within 2 weeks, down to levels close to those observed in BAFF-deficient mice. This depletion could then be maintained with a chronic treatment. Sandy-2 and a previously described rat IgG1 antibody, 5A8, also formed a pair suitable for the sensitive detection of endogenous circulating BAFF by ELISA or using a homogenous assay. Interestingly, 5A8 and Sandy-5 displayed activities opposite to that of Sandy-2 by stimulating recombinant BAFF in vitro and endogenous BAFF in vivo These tools will prove useful for the detection and functional manipulation of endogenous mouse BAFF and provide an alternative to the widely used BAFF receptor-Fc decoy receptor for the specific depletion of BAFF in mice.
Keywords
Animals, Antibodies/immunology, Antibodies/pharmacology, B-Cell Activating Factor/antagonists & inhibitors, B-Cell Activating Factor/genetics, B-Cell Activating Factor/immunology, B-Lymphocytes/immunology, B-Lymphocytes/pathology, Cell Survival/drug effects, Hyperplasia, Immunoglobulin G/immunology, Immunoglobulin G/pharmacology, Lymphocyte Depletion/methods, Mice, Mice, Knockout
Pubmed
Open Access
Yes
Create date
13/10/2016 13:11
Last modification date
20/08/2019 15:08