Development of bacteria-based bioassays for arsenic detection in natural waters.

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Serval ID
serval:BIB_52FEEDE4E201
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Development of bacteria-based bioassays for arsenic detection in natural waters.
Journal
Analytical and Bioanalytical Chemistry
Author(s)
Diesel E., Schreiber M., van der Meer J.R.
ISSN
1618-2650[electronic]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
394
Number
3
Pages
687-693
Language
english
Abstract
Arsenic contamination of natural waters is a worldwide concern, as the drinking water supplies for large populations can have high concentrations of arsenic. Traditional techniques to detect arsenic in natural water samples can be costly and time-consuming; therefore, robust and inexpensive methods to detect arsenic in water are highly desirable. Additionally, methods for detecting arsenic in the field have been greatly sought after. This article focuses on the use of bacteria-based assays as an emerging method that is both robust and inexpensive for the detection of arsenic in groundwater both in the field and in the laboratory. The arsenic detection elements in bacteria-based bioassays are biosensor-reporter strains; genetically modified strains of, e.g., Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Rhodopseudomonas palustris. In response to the presence of arsenic, such bacteria produce a reporter protein, the amount or activity of which is measured in the bioassay. Some of these bacterial biosensor-reporters have been successfully utilized for comparative in-field analyses through the use of simple solution-based assays, but future methods may concentrate on miniaturization using fiberoptics or microfluidics platforms. Additionally, there are other potential emerging bioassays for the detection of arsenic in natural waters including nematodes and clams.
Keywords
Arsenic/analysis, Bacteria/metabolism, Biological Assay/methods, Biosensing Techniques/methods, Fresh Water/chemistry
Pubmed
Web of science
Open Access
Yes
Create date
13/01/2010 14:27
Last modification date
01/10/2019 7:17
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