Glucose and lactate are equally effective in energizing activity-dependent synaptic vesicle turnover in purified cortical neurons.
Details
Serval ID
serval:BIB_52D625761B9E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Glucose and lactate are equally effective in energizing activity-dependent synaptic vesicle turnover in purified cortical neurons.
Journal
Neuroscience
ISSN
0306-4522
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
141
Number
1
Pages
157-65
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
This study examines the role of glucose and lactate as energy substrates to sustain synaptic vesicle cycling. Synaptic vesicle turnover was assessed in a quantitative manner by fluorescence microscopy in primary cultures of mouse cortical neurons. An electrode-equipped perfusion chamber was used to stimulate cells both by electrical field and potassium depolarization during image acquisition. An image analysis procedure was elaborated to select in an unbiased manner synaptic boutons loaded with the fluorescent dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl)pyridinium dibromide (FM1-43). Whereas a minority of the sites fully released their dye content following electrical stimulation, others needed subsequent K(+) depolarization to achieve full release. This functional heterogeneity was not significantly altered by the nature of metabolic substrates. Repetitive stimulation sequences of FM1-43 uptake and release were then performed in the absence of any metabolic substrate and showed that the number of active sites dramatically decreased after the first cycle of loading/unloading. The presence of 1 mM glucose or lactate was sufficient to sustain synaptic vesicle cycling under these conditions. Moreover, both substrates were equivalent for recovery of function after a phase of decreased metabolic substrate availability. Thus, lactate appears to be equivalent to glucose for sustaining synaptic vesicle turnover in cultured cortical neurons during activity.
Keywords
Animals, Cells, Cultured, Cerebral Cortex, Chi-Square Distribution, Diagnostic Imaging, Dose-Response Relationship, Drug, Drug Interactions, Embryo, Mammalian, Fluorescent Antibody Technique, Glucose, Lactic Acid, Mice, Neurons, Potassium, Pyridinium Compounds, Quaternary Ammonium Compounds, Synaptic Vesicles, Time Factors
Pubmed
Web of science
Create date
26/02/2008 16:25
Last modification date
20/08/2019 14:08