Protein protection by antioxidants: development of a convenient assay and structure-activity relationships of natural polyphenols

Details

Serval ID
serval:BIB_51B72075021C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Protein protection by antioxidants: development of a convenient assay and structure-activity relationships of natural polyphenols
Journal
Helvetica Chimica Acta
Author(s)
Salvi A., Bruhlmann C., Migliavacca E., Carrupt P.A., Hostettmann K., Testa B.
ISSN
0018-019X
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
85
Number
3
Pages
867-881
Language
english
Abstract
In this work, a convenient test of antioxidant activity was developed, with BChE-contaminated HSA as the target of AAPH-induced oxidation and its esterase activity as the marker of protein integrity or degradation. The method is relatively simple, of low cost, and convenient to use. Its application to natural polyphenols showed that quercetin (1), verbascoside (2), chlorogenic acid (3), caffeic acid (4),1,3,6,7-tetrahydroxyxanthone (5), and mangiferin (6), are good antioxidants (IC50 < 9 muM). 1,5-Dihydroxy-3-methoxyxanthone (7), flemichin D (8), and cordigone (9) showed modest activities (ca. 50 muM < IC50 < 350 muM), whereas danthrone (10) was inactive. Complementary experiments with two of the more active antioxidants, namely quercetin (1) and chlorogenic acid (3) showed that both antioxidants were better radical scavengers than chain-breaking antioxidants. The relative adiabatic oxidation potential (DeltaH(ox)), the relative H-bond dissociation energy (DeltaH(abs)), and the first oxidation potential measured by cyclic voltammetry were found to be related to the radical-scavenging activity of these antioxidants.
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Create date
08/04/2011 16:45
Last modification date
20/08/2019 14:07
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