Primary mouse keratinocyte cultures contain hair follicle progenitor cells with multiple differentiation potential.

Details

Serval ID
serval:BIB_51865ADBFAD3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Primary mouse keratinocyte cultures contain hair follicle progenitor cells with multiple differentiation potential.
Journal
Journal of Investigative Dermatology
Author(s)
Kamimura J., Lee D., Baden H.P., Brissette J., Dotto G.P.
ISSN
0022-202X (Print)
ISSN-L
0022-202X
Publication state
Published
Issued date
1997
Volume
109
Number
4
Pages
534-540
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
The interfollicular epidermis contains a single type of terminally differentiated keratinocytes, whereas hair follicles are composed of a minimum of six or seven distinct types. Whether or not these various populations of terminally differentiated keratinocytes originate from one or more progenitor cells has not been established. A related and important question is whether keratinocyte progenitor cells with a pluripotent potential, able to form not only epidermis but also hair follicles, can be maintained in vitro for any period of time. We have addressed these questions using skin reconstitution assays with admixed populations of genetically labeled, cultured keratinocytes. Examination of reconstituted epidermis and hair follicles showed that neither was composed of a random mixture of differently labeled keratinocytes, as would be predicted if they originated from a random reassociation of cells. Instead, the reconstituted interfollicular epidermis contained distinct columnar units, comprising all the overlying layers and most likely derived from a single progenitor cell. In contrast, hair follicles were found to be composed of cells of multiple origin, with each population showing a striking localization to a separate concentric region. The vast majority of reconstituted follicles appeared to derive from a minimum of two or, in a significant fraction of cases, three progenitor cells, one for the generation of the shaft (cuticle, cortex, and medulla), one for the inner root sheath, and the third for the outer root sheath. The general implications of these findings for epidermis and hair follicle formation and for keratinocyte stem cell cultivation are discussed.
Keywords
Animals, Cell Differentiation/physiology, Cell Line, Cells, Cultured, Epidermis/cytology, Epidermis/growth & development, Hair/growth & development, Hair Follicle/cytology, Haplotypes, Keratinocytes/cytology, Keratinocytes/physiology, Major Histocompatibility Complex/genetics, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Stem Cells/cytology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:58
Last modification date
08/05/2019 18:35
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