AtABCG29 is a monolignol transporter involved in lignin biosynthesis.

Details

Serval ID
serval:BIB_506A3DC7CE00
Type
Article: article from journal or magazin.
Collection
Publications
Title
AtABCG29 is a monolignol transporter involved in lignin biosynthesis.
Journal
Current Biology
Author(s)
Alejandro S., Lee Y., Tohge T., Sudre D., Osorio S., Park J., Bovet L., Lee Y., Geldner N., Fernie A.R., Martinoia E.
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Publication state
Published
Issued date
2012
Volume
22
Number
13
Pages
1207-1212
Language
english
Abstract
Lignin is the defining constituent of wood and the second most abundant natural polymer on earth. Lignin is produced by the oxidative coupling of three monolignols: p-coumaryl alcohol, coniferyl alcohol, and sinapyl alcohol. Monolignols are synthesized via the phenylpropanoid pathway and eventually polymerized in the cell wall by peroxidases and laccases. However, the mechanism whereby monolignols are transported from the cytosol to the cell wall has remained elusive. Here we report the discovery that AtABCG29, an ATP-binding cassette transporter, acts as a p-coumaryl alcohol transporter. Expression of AtABCG29 promoter-driven reporter genes and a Citrine-AtABCG29 fusion construct revealed that AtABCG29 is targeted to the plasma membrane of the root endodermis and vascular tissue. Moreover, yeasts expressing AtABCG29 exhibited an increased tolerance to p-coumaryl alcohol by excreting this monolignol. Vesicles isolated from yeasts expressing AtABCG29 exhibited a p-coumaryl alcohol transport activity. Loss-of-function Arabidopsis mutants contained less lignin subunits and were more sensitive to p-coumaryl alcohol. Changes in secondary metabolite profiles in abcg29 underline the importance of regulating p-coumaryl alcohol levels in the cytosol. This is the first identification of a monolignol transporter, closing a crucial gap in our understanding of lignin biosynthesis, which could open new directions for lignin engineering.
Pubmed
Web of science
Open Access
Yes
Create date
18/01/2013 17:13
Last modification date
08/05/2019 18:31
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