Noradrenaline enhances monocarboxylate transporter 2 expression in cultured mouse cortical neurons via a translational regulation

Details

Serval ID
serval:BIB_50684B403200
Type
Article: article from journal or magazin.
Collection
Publications
Title
Noradrenaline enhances monocarboxylate transporter 2 expression in cultured mouse cortical neurons via a translational regulation
Journal
Journal of Neurochemistry
Author(s)
Pierre  K., Debernardi  R., Magistretti  P. J., Pellerin  L.
ISSN
0022-3042 (Print)
Publication state
Published
Issued date
09/2003
Volume
86
Number
6
Pages
1468-76
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
Regulation of the expression of MCT1 and MCT2, two isoforms of the monocarboxylate transporter (MCT) family, was investigated in primary cultures of mouse cortical neurons. Under basal conditions, both MCT immunoreactivities (IR) were found in the cell soma and dendrites, although IR for MCT1 appeared less bright than for MCT2. Treatment of cultured cortical neurons with 100 microm noradrenaline (NA) led, after a few hours, to a striking enhancement in fluorescence intensity associated with MCT2 IR in the cell soma as well as in dendrites. In contrast, MCT1 IR was not altered by NA treatment. Western blot experiments performed on cultured neurons treated with NA confirmed that MCT2 protein expression was increased. Forskolin and dBcAMP also enhanced MCT2 expression, suggesting the implication of a cAMP-mediated pathway in the effect of NA. Surprisingly, neither NA, dBcAMP nor forskolin affected MCT2 mRNA expression. Application of cycloheximide, a protein synthesis inhibitor, prevented the enhancement of MCT2 IR, while the mRNA synthesis inhibitor actinomycin D also blocked the effect of NA on MCT2 IR levels. These results suggest that regulation of MCT2 expression in neurons by NA occurs at the translational level despite the requirement for an as yet unknown transcriptional step.
Keywords
Animals Blotting, Western Bucladesine/pharmacology Cells, Cultured Cerebral Cortex/cytology Forskolin/pharmacology Gene Expression Regulation/drug effects/*physiology Immunohistochemistry Mice Monocarboxylic Acid Transporters/genetics/*metabolism Neurons/cytology/drug effects/*metabolism Norepinephrine/*pharmacology Nucleic Acid Synthesis Inhibitors/pharmacology Protein Biosynthesis/*drug effects Protein Synthesis Inhibitors/pharmacology RNA, Messenger/metabolism Symporters/genetics/metabolism
Pubmed
Web of science
Create date
24/01/2008 14:17
Last modification date
03/03/2018 17:08
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