Expression of hepatitis C virus proteins does not interfere with major histocompatibility complex class I processing and presentation in vitro

Details

Serval ID
serval:BIB_4FC3E91C1191
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Expression of hepatitis C virus proteins does not interfere with major histocompatibility complex class I processing and presentation in vitro
Journal
Hepatology
Author(s)
Moradpour  D., Grabscheid  B., Kammer  A. R., Schmidtke  G., Groettrup  M., Blum  H. E., Cerny  A.
ISSN
0270-9139 (Print)
Publication state
Published
Issued date
05/2001
Volume
33
Number
5
Pages
1282-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Hepatitis C virus (HCV) infection takes a chronic course in the majority of patients. The mechanisms underlying the evasion of the host immune response and viral persistence are poorly understood. In this context, we investigated interactions of HCV proteins with major histocompatibility complex (MHC) class I processing and presentation pathways using cell lines that allow the tetracycline-regulated expression of viral structural and nonstructural proteins. These well-characterized inducible cell lines were found to efficiently process and present endogenously synthesized HCV proteins via MHC class I. Functional MHC class I cell-surface expression and intracellular proteasome activity were not affected by the expression of HCV proteins. These results suggest that viral evasion of the host immune response does not involve interactions of HCV with MHC class I processing and presentation. Other mechanisms, such as interference with the interferon system, may be operative in HCV infection, leading to viral persistence.
Keywords
Cell Membrane/metabolism Cysteine Endopeptidases/physiology HLA Antigens/classification HLA-A2 Antigen/immunology Hepacivirus/*metabolism Histocompatibility Antigens Class I/*metabolism Humans Intracellular Membranes/physiology Multienzyme Complexes/physiology Proteasome Endopeptidase Complex *Protein Processing, Post-Translational T-Lymphocytes, Cytotoxic/immunology/physiology Tumor Cells, Cultured Viral Proteins/*metabolism/physiology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:06
Last modification date
20/08/2019 14:05
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