Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L)

Details

Serval ID
serval:BIB_4FB173DBDDB3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L)
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Huang  D. C., Hahne  M., Schroeter  M., Frei  K., Fontana  A., Villunger  A., Newton  K., Tschopp  J., Strasser  A.
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
12/1999
Volume
96
Number
26
Pages
14871-6
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 21
Abstract
Fas activation triggers apoptosis in many cell types. Studies with anti-Fas antibodies have produced conflicting results on Fas signaling, particularly the role of the Bcl-2 family in this process. Comparison between physiological ligand and anti-Fas antibodies revealed that only extensive Fas aggregation, by membrane bound FasL or aggregated soluble FasL consistently triggered apoptosis, whereas antibodies could act as death agonists or antagonists. Studies on Fas signaling in cell lines and primary cells from transgenic mice revealed that FADD/MORT1 and caspase-8 were required for apoptosis. In contrast, Bcl-2 or Bcl-x(L) did not block FasL-induced apoptosis in lymphocytes or hepatocytes, demonstrating that signaling for cell death induced by Fas and the pathways to apoptosis regulated by the Bcl-2 family are distinct.
Keywords
*Adaptor Proteins, Signal Transducing Animals Antigens, CD95/immunology/*metabolism Apoptosis/*physiology Biological Transport Carrier Proteins/metabolism Caspase 8 Caspase 9 Caspases/metabolism Cell Membrane/*metabolism Fas Ligand Protein Fas-Associated Death Domain Protein Immunologic Capping Liver/cytology/physiology Lymphocytes/physiology Membrane Glycoproteins/*metabolism Mice Mice, Transgenic Protein Precursors/metabolism Proto-Oncogene Proteins c-bcl-2/*metabolism Signal Transduction bcl-X Protein
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 14:05
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