Modulation of endoplasmic reticulum calcium pump expression during lung cancer cell differentiation.

Details

Serval ID
serval:BIB_4F6314973A2E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Modulation of endoplasmic reticulum calcium pump expression during lung cancer cell differentiation.
Journal
Febs Journal
Author(s)
Arbabian A., Brouland J.P., Apáti Á., Pászty K., Hegedűs L., Enyedi Á., Chomienne C., Papp B.
ISSN
1742-4658 (Electronic)
ISSN-L
1742-464X
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
280
Number
21
Pages
5408-5418
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Cellular calcium signaling plays important roles in several signal transduction pathways that control proliferation, differentiation and apoptosis. In epithelial cells calcium signaling is initiated mainly by calcium release from endoplasmic-reticulum-associated intracellular calcium pools. Because calcium is accumulated in the endoplasmic reticulum by sarco/endoplasmic reticulum calcium ATPases (SERCA), these enzymes play a critical role in the control of calcium-dependent cell activation, growth and survival. We investigated the modulation of SERCA expression and function in human lung adenocarcinoma cells. In addition to the ubiquitous SERCA2 enzyme, the SERCA3 isoform was also expressed at variable levels. SERCA3 expression was selectively enhanced during cell differentiation in lung cancer cells, and marked SERCA3 expression was found in fully differentiated normal bronchial epithelium. As studied by using a recombinant fluorescent calcium probe, induction of the expression of SERCA3, a lower calcium affinity pump, was associated with decreased intracellular calcium storage, whereas the amplitude of capacitative calcium influx remained unchanged. Our observations indicate that the calcium homeostasis of the endoplasmic reticulum in lung adenocarcinoma cells presents a functional defect due to decreased SERCA3 expression that is corrected during pharmacologically induced differentiation. The data presented in this work show, for the first time, that endoplasmic reticulum calcium storage is anomalous in lung cancer cells, and suggest that SERCA3 may serve as a useful new phenotypic marker for the study of lung epithelial differentiation.
Keywords
Adenocarcinoma/metabolism, Adenocarcinoma/pathology, Blotting, Western, Calcium Signaling, Cell Differentiation, Cell Proliferation, Endoplasmic Reticulum/enzymology, Homeostasis, Humans, Immunoenzyme Techniques, Lung Neoplasms/metabolism, Lung Neoplasms/pathology, Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
13/10/2015 10:14
Last modification date
20/08/2019 14:05
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