Multiple specificities in the repertoire of a melanoma patient's cytolytic T lymphocytes directed against tumor antigen MAGE-1.A1

Details

Serval ID
serval:BIB_4E969907676D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Multiple specificities in the repertoire of a melanoma patient's cytolytic T lymphocytes directed against tumor antigen MAGE-1.A1
Journal
Journal of Experimental Medicine
Author(s)
Romero  P., Pannetier  C., Herman  J., Jongeneel  C. V., Cerottini  J. C., Coulie  P. G.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
10/1995
Volume
182
Number
4
Pages
1019-28
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Abstract
Peptide MAGE-1.A1 is a nonamer derived from protein MAGE-1 that can associate with the HLA-A1 molecule. It was shown previously to be recognized by an antitumor cytolytic T lymphocyte (CTL) clone derived from the blood of melanoma patient MZ2. We derived two other anti-MAGE-1.A1 CTL clones from different blood samples of the same patient and compared the fine specificity of recognition of the three CTL by testing them on variant MAGE-1.A1 peptides incorporating different amino acid substitutions. The epitopes recognized by the CTL proved to be different. While modifications of residues at positions 5, 6, or 7 in the antigenic peptide affected recognition by the three CTL, each of the modifications of residues at positions 1, 4, or 8 affected recognition by one CTL only. The sequences of both the alpha and beta chains of the T cell antigen receptor of the three CTL were completely different. The results indicate a long-lasting diversity in terms of fine specificity and of T cell antigen receptor structure in the repertoire of antitumor CTL derived from the blood of a melanoma patient and directed against a defined tumor antigen.
Keywords
Amino Acid Sequence Antigens, Neoplasm/*immunology Base Sequence Clone Cells Cloning, Molecular Female Humans Melanoma/*immunology Molecular Sequence Data *Neoplasm Proteins Oligopeptides/*immunology Receptors, Antigen, T-Cell, alpha-beta/genetics/*immunology Sequence Analysis, DNA Sequence Homology, Amino Acid Structure-Activity Relationship T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:39
Last modification date
20/08/2019 14:04
Usage data