Distribution of intermediate filament proteins in normal and diseased human glomeruli.

Details

Serval ID
serval:BIB_4E55A8218CC5
Type
Article: article from journal or magazin.
Collection
Publications
Title
Distribution of intermediate filament proteins in normal and diseased human glomeruli.
Journal
American Journal of Pathology
Author(s)
Stamenkovic I., Skalli O., Gabbiani G.
ISSN
0002-9440[print], 0002-9440[linking]
Publication state
Published
Issued date
1986
Volume
125
Number
3
Pages
465-475
Language
english
Notes
Publication types: Journal Article
Abstract
The distribution of intermediate filament proteins (vimentin, desmin, and cytokeratin) was studied by means of immunofluorescence in the normal human and rat glomerulus and in pathologic human glomeruli. Antifibronectin antibodies were used as mesangial markers. In normal human glomeruli, vimentin antibodies stained endothelial cells, podocytes, and mesangial cells; desmin antibodies, surprisingly, stained podocytes. In normal rat glomeruli, the pattern of vimentin staining was the same as in humans, but desmin antibodies stained both mesangial cells and podocytes. In human and rat glomeruli cytokeratin staining was confined to segments of Bowman's capsule. In human pathologic glomeruli, vimentin and desmin antibodies stained the structures that were positive in normal glomeruli, giving a characteristic pattern for each pathologic condition examined. These results are compatible with the mesenchymal origin of podocytes and mesangial cells and suggest that both cells have smooth muscle-like phenotypic features. Mesangial cells may have slightly different differentiation paths in humans and rats, leading to a distinct expression of intermediate filament proteins.
Keywords
Animals, Desmin/metabolism, Female, Fibronectins/immunology, Fluorescent Antibody Technique, Glomerulonephritis/metabolism, Humans, Intermediate Filament Proteins/metabolism, Keratins/metabolism, Kidney Glomerulus/metabolism, Rats, Vimentin/metabolism
Pubmed
Create date
25/08/2010 14:54
Last modification date
20/08/2019 14:03
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