A pentaplex automated fluorescent typing system for forensic identification and French Caucasian population data.

Details

Serval ID
serval:BIB_4E2EB391B464
Type
Article: article from journal or magazin.
Collection
Publications
Title
A pentaplex automated fluorescent typing system for forensic identification and French Caucasian population data.
Journal
Journal of Forensic Sciences
Author(s)
Rousselet F., Pfitzinger H., Mangin P.
ISSN
0022-1198[print], 0022-1198[linking]
Publication state
Published
Issued date
1997
Volume
42
Number
3
Pages
500-503
Language
english
Abstract
The polymerase chain reaction (PCR) amplification of short tandem repeat (STR) loci has already proven to be a method of choice for large scale typing of DNA samples in which the conventional restriction fragment length polymorphism (RFLP) technique is ineffective. A quadruplex PCR including HUMvWFA31A, HUMF13A01, HUMTH01, and HUMFESFPS STR loci is used successfully for routine forensic applications in our laboratory. However, the need to increase the discrimination power of the PCR systems used prompted us to develop a second system of a pentaplex PCR for the analysis of 4 additional STR loci (HUMD8S1179, HUMD18S51, HUMD21S11, and HUMFIBRA) and the sex determination by amplification of a segment of the X-Y homologous Amelogenin gene. Allele and phenotype frequencies for these 4 STR systems were obtained by multiplex amplification, from approximately 200 randomly selected and unrelated French Caucasian individuals. Statistical calculations for these phenotype distributions met expectations for Hardy-Weinberg equilibrium. Furthermore, the French allelic frequencies of D18S51, D21S11, and HUMFIBRA loci were compared with the data obtained by the Forensic Science Service (UK) for the British Caucasian population and proved to be similar.
Keywords
Alleles, Amelogenin, Dental Enamel Proteins/genetics, European Continental Ancestry Group/genetics, Female, Fluorescent Dyes, Forensic Medicine/methods, France, Genetic Heterogeneity, Humans, Male, Polymerase Chain Reaction/methods, Repetitive Sequences, Nucleic Acid, Tooth Germ
Pubmed
Create date
19/10/2010 16:14
Last modification date
20/08/2019 14:03
Usage data