Altered growth and phenotype in clonal mycN transfectants of the SK-N-SH neuroblastoma cell line

Details

Serval ID
serval:BIB_4E185CDE2F99
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Altered growth and phenotype in clonal mycN transfectants of the SK-N-SH neuroblastoma cell line
Journal
International Journal of Cancer
Author(s)
Gross  N., Miescher  G., Beck  D., Favre  S., Beretta  C.
ISSN
0020-7136
Publication state
Published
Issued date
10/1994
Peer-reviewed
Oui
Volume
59
Number
1
Pages
141-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Abstract
We have attempted to distinguish in human neuroblastoma between the effects of mycN on differentiation and its potential to promote malignant progression. Others have observed out-growth of autocrine cells with evidence of an advanced malignant phenotype in a mycN-transfected clonal cell line derived from the single-copy mycN neuroblastoma, SK-N-SH. We have now transfected the parental cell line with the same mycN expression vector and selected 5 clones characterized by unique and stable chromosomal integration sites and variable exogenous copy numbers. mycN gene expression was variable in the different clones and correlated roughly with the copy number of transfected mycN genes. Clones with minimal levels of mycN gene expression had a neuroblastic phenotype and low numbers of surface HLA class-I molecules. Clones with high levels of mycN expression had a Schwann/glial-like phenotype with higher surface HLA class-I display without imbalance of expression of specific loci and accelerated growth. Two such clones were capable of anchorage-independent growth in the absence of serum, and acquired tumorigenic properties. Our results show that exogenous mycN expression can be associated with a differentiation of neuroblastoma cells along the Schwann/glial pathway and can induce accelerated and autonomous growth.
Keywords
Animals Blotting, Northern Blotting, Southern Cell Division Gene Expression *Genes, myc Genetic Vectors Histocompatibility Antigens Class I/analysis Kinetics Mice Mice, Nude Neoplasm Transplantation Neuroblastoma/*pathology *Phenotype RNA, Messenger/metabolism S100 Proteins/analysis *Transfection Tumor Cells, Cultured Vimentin/analysis
Pubmed
Web of science
Create date
20/01/2008 15:55
Last modification date
20/08/2019 14:03
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