The immunogenicity of antigen bound to the plasma membrane of macrophages.

Details

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State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_4E13176F72D8
Type
Article: article from journal or magazin.
Collection
Publications
Title
The immunogenicity of antigen bound to the plasma membrane of macrophages.
Journal
The Journal of experimental medicine
Author(s)
Unanue E.R., Cerottini J.C.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
01/04/1970
Peer-reviewed
Oui
Volume
131
Number
4
Pages
711-725
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Macrophages were cultured for several hours after a brief exposure to radio-iodinated keyhole limpet hemocyanin. Most of the hemocyanin taken up by the macrophages was rapidly catabolized and eliminated from the cell. A few molecules were retained on the plasma membrane of the cells for prolonged periods and were not subject to endocytosis and catabolism. These few molecules of hemocyanin bound to the plasma membrane were identified by observing the fixation of antibody fragments to macrophages at low temperature. The membrane-bound antigen, which could be removed by trypsin or EDTA, was of large molecular size, though heterogeneous. A great part of the immune responses of mice to hemocyanin bound to live macrophages could be abrogated by treatment of the macrophages in vitro with antibody or trypsin. Hence, most of the immunogenicity of hemocyanin bound to macrophages was attributed to the few molecules of antigen bound to the plasma membrane.
Keywords
Animals, Antibody Formation, Antigen-Antibody Reactions, Antigens/analysis, Binding Sites, Cell Membrane/immunology, Cell Membrane/metabolism, Hemocyanins/pharmacology, Immunoassay, Iodine Isotopes, Macrophages/drug effects, Macrophages/immunology, Mice
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:13
Last modification date
09/08/2024 12:40
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