Gene polymorphisms for elucidating the genetic structure of the heavy-metal hyperaccumulating trait in Thlaspi caerulescens and their cross-genera amplification in Brassicaceae.

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Type
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Title
Gene polymorphisms for elucidating the genetic structure of the heavy-metal hyperaccumulating trait in Thlaspi caerulescens and their cross-genera amplification in Brassicaceae.
Journal
Journal of Plant Research
Author(s)
Basic N., Besnard G.
ISSN
0918-9440 (Print)
ISSN-L
0918-9440
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
119
Number
5
Pages
479-487
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Genetic polymorphism was investigated in Thlaspi caerulescens J. & C. Presl at 15 gene regions, of which seven have been identified to putatively play a role in heavy-metal tolerance or hyperaccumulation. Single nucleotide and length polymorphisms were assessed at four cleaved amplified polymorphic sequences (CAPS) and 11 simple sequence repeat (microsatellite) loci, respectively. The utility of these loci for genetic studies in T. caerulescens was measured among seven natural populations (135 individuals). Fourteen loci rendered polymorphism, and the number of alleles per locus varied from 2 to 5 and 1 to 27 for CAPS and microsatellites, respectively. Up to 12 alleles per locus were detected in a population. The global observed heterozygosity per population varied between 0.01 and 0.31. Additionally, cross-species/genera amplification of loci was investigated on eight other Brassicaceae (five individuals per population). Overall, 70% of the cross-species/genera amplifications were successful, and among them, more than 40% provided intraspecific polymorphisms within a single population. This indicates that such markers may, as well, allow comparative population genetic or mapping studies between and within several Brassicaceae, particularly for genes involved in traits such as heavy-metal tolerance and/or hyperaccumulation.
Keywords
Alleles, DNA, Plant/metabolism, Gene Amplification, Gene Frequency, Genetic Markers, Genome, Plant/genetics, Metals, Heavy/metabolism, Microsatellite Repeats/genetics, Polymerase Chain Reaction, Polymorphism, Genetic/genetics, Thlaspi/genetics, Thlaspi/metabolism
Pubmed
Web of science
Create date
20/01/2008 15:17
Last modification date
20/08/2019 14:03
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