Early effects of aldosterone on apical and basolateral membrane conductances of TBM cells

Details

Serval ID
serval:BIB_4DE2D663A3AF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Early effects of aldosterone on apical and basolateral membrane conductances of TBM cells
Journal
American Journal of Physiology
Author(s)
Horisberger  J. D.
ISSN
0002-9513 (Print)
Publication state
Published
Issued date
08/1992
Volume
263
Number
2 Pt 1
Pages
C384-8
Notes
Journal Article --- Old month value: Aug
Abstract
The early effects (1-4 h) of aldosterone on membrane conductance were studied in the TBM cells, an epithelial cell line derived from the toad urinary bladder. Aldosterone stimulated Na+ transport (measured as short-circuit current) and induced parallel increases of the amiloride-sensitive apical membrane Na+ conductance and the basolateral membrane conductance. In another set of experiments, we prevented the aldosterone-induced increase of the Na+ transport by adding amiloride to the apical solution in concentrations sufficient to reduce short-circuit current to values similar to that of matched control. The effect of aldosterone on the basolateral conductance was not inhibited. This effect was essentially due to a change of the barium-sensitive K(+)-specific conductance. We also observed that aldosterone prevented a time-dependent decline of the paracellular resistance observed in control preparations. Aldosterone induces an early increase of the basolateral membrane conductance, an effect that is not secondary to the increase of the Na+ load to the cell.
Keywords
Aldosterone/*pharmacology Animals Cell Line Cell Membrane/physiology Electric Conductivity Epithelial Cells Epithelium/physiology Membrane Potentials/drug effects Potassium/physiology Sodium/physiology Urinary Bladder/cytology/*physiology
Pubmed
Web of science
Create date
24/01/2008 12:38
Last modification date
20/08/2019 14:03
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