Unconjugated TAT carrier peptide protects against excitotoxicity.

Details

Serval ID
serval:BIB_4DC6866BE3EF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Unconjugated TAT carrier peptide protects against excitotoxicity.
Journal
Neurotoxicity Research
Author(s)
Vaslin A., Rummel C., Clarke P.G.
ISSN
1476-3524 (Electronic)
ISSN-L
1029-8428
Publication state
Published
Issued date
2009
Volume
15
Number
2
Pages
123-126
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
We report in this article for the first time the neuroprotective effects of unconjugated TAT carrier peptide against a mild excitotoxic stimulus both in vitro and in vivo. In view of the widespread use of TAT peptides to deliver neuroprotectants into cells, it is important to know the effects of the carrier itself. Unconjugated TAT carrier protects dissociated cortical neurons against NMDA but not against kainate, suggesting that TAT peptides may interfere with NMDA signaling. Furthermore, a retro-inverso form of the carrier peptide caused a reduction in lesion volume (by about 50%) in a rat neonatal cerebral ischemia model. Thus, even though TAT is designed merely as a carrier, its own pharmacological activity will need to be considered in the analysis of TAT-linked neuroprotectant peptides.
Keywords
Age Factors, Animals, Animals, Newborn, Brain Ischemia/prevention & control, Cell Death/drug effects, Cells, Cultured, Cerebral Cortex/cytology, Disease Models, Animal, Dizocilpine Maleate/pharmacology, Dose-Response Relationship, Drug, Excitatory Amino Acid Agonists/pharmacology, Kainic Acid/pharmacology, L-Lactate Dehydrogenase/metabolism, N-Methylaspartate/pharmacology, Neurons/drug effects, Neuroprotective Agents/pharmacology, Rats, Rats, Sprague-Dawley, Time Factors, tat Gene Products, Human Immunodeficiency Virus/pharmacology
Pubmed
Web of science
Create date
17/03/2009 10:56
Last modification date
20/08/2019 14:02
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