Inducible gene and shRNA expression in resident hematopoietic stem cells in vivo.

Details

Serval ID
serval:BIB_4D8E797BD6F1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inducible gene and shRNA expression in resident hematopoietic stem cells in vivo.
Journal
Stem Cells
Author(s)
Laurenti E., Barde I., Verp S., Offner S., Wilson A., Quenneville S., Wiznerowicz M., Macdonald H.R., Trono D., Trumpp A.
ISSN
1549-4918[electronic], 1066-5099[linking]
Publication state
Published
Issued date
2010
Volume
28
Number
8
Pages
1390-1398
Language
english
Abstract
Hematopoietic stem cells (HSC) are probably the best understood somatic stem cells and often serve as a paradigm for other stem cells. Nevertheless, most current techniques to genetically manipulate them in vivo are either constitutive and/or induced in settings of hematopoietic stress such as after irradiation. Here, we present a conditional expression system that allows for externally controllable transgenesis and knockdown in resident HSCs, based on a lentiviral vector containing a tet-O sequence and a transgenic mouse line expressing a doxycyclin-regulated tTR-KRAB repressor protein. HSCs harvested from tTR-KRAB mice are transduced with the lentiviral vector containing a cDNA (i.e., Green Fluorescent Protein (GFP)) and/or shRNA (i.e., p53) of interest and then transplanted into lethally irradiated recipients. While the vector is effectively repressed by tTR-KRAB during homing and engraftment, robust GFP/shp53 expression is induced on doxycyclin treatment in HSCs and their progeny. Doxycylin-controllable transcription is maintained on serial transplantation, indicating that repopulating HSCs are stably modified by this approach. In summary, this easy to implement conditional system provides inducible and reversible overexpression or knock down of genes in resident HSCs in vivo using a drug devoid of toxic or activating effects.
Keywords
Animals, Apoptosis/drug effects, Apoptosis/genetics, Cell Cycle/drug effects, Cell Cycle/genetics, Cell Proliferation, Cells, Cultured, Doxycycline/pharmacology, Female, Flow Cytometry, Gene Expression/drug effects, Gene Expression/genetics, Genetic Vectors/genetics, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/drug effects, Humans, Lentivirus/genetics, Mice, Mice, Inbred C57BL, Mice, Transgenic, RNA, Small Interfering/genetics, RNA, Small Interfering/physiology, Reverse Transcriptase Polymerase Chain Reaction, Tumor Suppressor Protein p53/genetics
Pubmed
Web of science
Open Access
Yes
Create date
02/02/2011 9:57
Last modification date
20/08/2019 14:02
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