The Tightness of the Cotton Swabs Meshing Influences the Chances of Getting Conclusive DNA Profiles

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Version: author
Serval ID
serval:BIB_4D75BE0766CF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Tightness of the Cotton Swabs Meshing Influences the Chances of Getting Conclusive DNA Profiles
Journal
Journal of Forensic Research
Author(s)
Rocque MJ, Leake SL, Milon MP, Castella V.
ISSN
2157-7145
ISSN-L
2157-7145
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
5
Number
4
Pages
234-239
Language
english
Abstract
Objective: The chance of obtaining a conclusive DNA profile strongly depends on the quantity of biological material that can be recovered from a crime scene sample. Optimizing the collection strategy is therefore of prime interest. A difference in the level of tightness of the cotton meshed around the shaft has been observed between manufacturers and is hypothesized to affect the collection and subsequent release capacity of cotton swabs. Consequently, we compared the performance of cotton swabs from two different suppliers: Applimed SA and DryswabTM. Methods: These swabs were used to recover 50 ml of blood, either pure or diluted (1:1000 and 1:5000), deposited on both smooth and absorbent surfaces. Performance was compared in terms of ease of use, concentration of extracted DNA, and quality of DNA profiles. DNA quantification was obtained by real-time PCR using the QuantifilerTM Human DNA Quantification Kit. Evaluation of DNA profiles was based on profiles obtained using AmpFlSTR® NGM SElectTM PCR Amplification kit. Results: When considering smooth surfaces, recovered DNA was more concentrated when using the DryswabTM than the Applimed SA cotton swab. More precisely, DNA concentrations ranged from 15.7 to 28.8 ng/ml and 6.7 to 21.2 ng/ml, respectively for samples of pure blood. The same trend was observed for the absorbent surface, with 2.0 to 5.0 ng/ml and 0.9 to 1.4 ng/ml, respectively. Conclusion: Our results illustrate that different cotton swabs produce different results in terms of ease of use and quantity of recovered DNA and this should be taken into consideration when choosing which swab to use at both the crime scene and laboratory. More specifically, results from the present study suggest that looser meshing of the cotton fibres
Create date
18/09/2014 11:30
Last modification date
20/08/2019 15:02
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