Cloning of two human homologs of the Drosophila single-minded gene SIM1 on chromosome 6q and SIM2 on 21q within the Down syndrome chromosomal region.

Details

Serval ID
serval:BIB_4D541786E6CC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cloning of two human homologs of the Drosophila single-minded gene SIM1 on chromosome 6q and SIM2 on 21q within the Down syndrome chromosomal region.
Journal
Genome Research
Author(s)
Chrast R., Scott H.S., Chen H., Kudoh J., Rossier C., Minoshima S., Wang Y., Shimizu N., Antonarakis S.E.
ISSN
1088-9051 (Print)
ISSN-L
1088-9051
Publication state
Published
Issued date
1997
Volume
7
Number
6
Pages
615-624
Language
english
Abstract
As part of our effort to clone genes of human chromosome 21 that may contribute to Down syndrome, we have previously isolated four exons with homology to Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of fruit fly neurogenesis. These exons were used to clone and characterize two human homologs of the Drosophila sim gene, SIM1 and SIM2, which map to chromosomes 6q16.3-q21 and 21q22.2, respectively; SIM2 maps within the so-called Down syndrome chromosomal region. Recently, two mouse homologs, Sim1 and Sim2, also have been identified. There is a high level of homology among human, mouse, and Drosophila sim genes in their amino-terminal half where the conserved bHLH, PAS1, PAS2, and HST domains are present. In contrast, the carboxy-terminal parts are only homologous between SIM1 and Sim1 and SIM2 and Sim2. Two isoforms (SIM2 and SIM2s) of human SIM2 have been detected that differ in their 3' ends. Northern blot analysis revealed one mRNA SIM1 species of approximately 9.5 kb and four different mRNA SIM2 species of 2.7, 3, 4.4, and 6 kb in human fetal kidney. The function of both human SIM1 and SIM2 is unknown. However, three copies of SIM2 may contribute to some specific Down syndrome phenotypes because of (1) mapping position, (2) potential function as transcriptional repressor, (3) likely dimerization with other transcription factors, (4) the temporal and spatial expression pattern of mouse Sim2, and (5) the potentially analogous role of human SIM2 to that of Drosophila sim during neurogenesis.
Keywords
Amino Acid Sequence, Animals, Basic Helix-Loop-Helix Transcription Factors, Chromosome Mapping, Chromosomes, Human, Pair 21, Chromosomes, Human, Pair 6, Cloning, Molecular, DNA-Binding Proteins/genetics, Down Syndrome/genetics, Drosophila/genetics, Drosophila Proteins, Humans, Mice, Molecular Sequence Data, Nuclear Proteins/genetics, Polymerase Chain Reaction, Repressor Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid
Pubmed
Web of science
Create date
24/01/2008 14:12
Last modification date
20/08/2019 14:02
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