Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria.

Details

Ressource 1Download: journal.pgen.1006499.pdf (5346.43 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_4D1D6B2F64F8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cell Cycle Constraints and Environmental Control of Local DNA Hypomethylation in α-Proteobacteria.
Journal
PLoS Genetics
Author(s)
Ardissone S., Redder P., Russo G., Frandi A., Fumeaux C., Patrignani A., Schlapbach R., Falquet L., Viollier P.H.
ISSN
1553-7404 (Electronic)
ISSN-L
1553-7390
Publication state
Published
Issued date
2016
Volume
12
Number
12
Pages
e1006499
Language
english
Abstract
Heritable DNA methylation imprints are ubiquitous and underlie genetic variability from bacteria to humans. In microbial genomes, DNA methylation has been implicated in gene transcription, DNA replication and repair, nucleoid segregation, transposition and virulence of pathogenic strains. Despite the importance of local (hypo)methylation at specific loci, how and when these patterns are established during the cell cycle remains poorly characterized. Taking advantage of the small genomes and the synchronizability of α-proteobacteria, we discovered that conserved determinants of the cell cycle transcriptional circuitry establish specific hypomethylation patterns in the cell cycle model system Caulobacter crescentus. We used genome-wide methyl-N6-adenine (m6A-) analyses by restriction-enzyme-cleavage sequencing (REC-Seq) and single-molecule real-time (SMRT) sequencing to show that MucR, a transcriptional regulator that represses virulence and cell cycle genes in S-phase but no longer in G1-phase, occludes 5'-GANTC-3' sequence motifs that are methylated by the DNA adenine methyltransferase CcrM. Constitutive expression of CcrM or heterologous methylases in at least two different α-proteobacteria homogenizes m6A patterns even when MucR is present and affects promoter activity. Environmental stress (phosphate limitation) can override and reconfigure local hypomethylation patterns imposed by the cell cycle circuitry that dictate when and where local hypomethylation is instated.

Pubmed
Web of science
Open Access
Yes
Create date
16/02/2017 17:05
Last modification date
20/08/2019 15:01
Usage data