TIE-2-expressing monocytes are lymphangiogenic and associate specifically with lymphatics of human breast cancer.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_4C97ACD28497
Type
Article: article from journal or magazin.
Collection
Publications
Title
TIE-2-expressing monocytes are lymphangiogenic and associate specifically with lymphatics of human breast cancer.
Journal
Oncoimmunology
Author(s)
Bron S., Henry L., Faes-Van't Hull E., Turrini R., Vanhecke D., Guex N., Ifticene-Treboux A., Marina Iancu E., Semilietof A., Rufer N., Lehr H.A., Xenarios I., Coukos G., Delaloye J.F., Doucey M.A.
ISSN
2162-4011 (Print)
ISSN-L
2162-4011
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
5
Number
2
Pages
e1073882
Language
english
Abstract
In experimental mouse models of cancer, increasingly compelling evidence point toward a contribution of tumor associated macrophages (TAM) to tumor lymphangiogenesis. Corresponding experimental observations in human cancer remain scarce although lymphatic metastasis is widely recognized as a predominant route for tumor spread. We previously showed that, in malignant tumors of untreated breast cancer (BC) patients, TIE-2-expressing monocytes (TEM) are highly proangiogenic immunosuppressive cells and that TIE-2 and VEGFR signaling pathways drive TEM immunosuppressive function. We report here that, in human BC, TEM express the canonical lymphatic markers LYVE-1, Podoplanin, VEGFR-3 and PROX-1. Critically, both TEM acquisition of lymphatic markers and insertion into lymphatic vessels were observed in tumors but not in adjacent non-neoplastic tissues, suggesting that the tumor microenvironment shapes both TEM phenotype and spatial distribution. We assessed the lymphangiogenic activity of TEM isolated from dissociated primary breast tumors in vitro and in vivo using endothelial cells (EC) sprouting assay and corneal vascularization assay, respectively. We show that, in addition to their known hemangiogenic function, TEM isolated from breast tumor display a lymphangiogenic activity. Importantly, TIE-2 and VEGFR pathways display variable contributions to TEM angiogenic and lymphangiogenic activities across BC patients; however, combination of TIE-2 and VEGFR kinase inhibitors abrogated these activities and overcame inter-patient variability. These results highlight the direct contribution of tumor TEM to the breast tumor lymphatic network and suggest a combined use of TIE-2 and VEGFR kinase inhibitors as a therapeutic approach to block hem- and lymphangiogenesis in BC.
Pubmed
Web of science
Create date
01/05/2016 17:00
Last modification date
20/08/2019 14:01
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