Lox10, a member of the NK-2 homeobox gene class, is expressed in a segmental pattern in the endoderm and in the cephalic nervous system of the leech Helobdella

Details

Serval ID
serval:BIB_4C81634A0506
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Lox10, a member of the NK-2 homeobox gene class, is expressed in a segmental pattern in the endoderm and in the cephalic nervous system of the leech Helobdella
Journal
Development
Author(s)
Nardelli-Haefliger  D., Shankland  M.
ISSN
0950-1991 (Print)
Publication state
Published
Issued date
07/1993
Volume
118
Number
3
Pages
877-92
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul
Abstract
A novel leech homeobox gene, Lox10, is shown to encode a homeodomain sequence characteristic of a phyletically widespread NK-2 homeobox gene class. Lox10 expression was examined in leech embryos of various ages by in situ hybridization. In the unsegmented cephalic region, Lox10 RNA is expressed in a subset of the cells descended from the a' and b' micromeres, including a small cluster of cells, believed to be postmitotic neurons, within the supraesophageal ganglion of the central nervous system. Hybridization signal was not detected in either the mesoderm or ectoderm of the trunk segments, and the apparent restriction of Lox10 ectodermal expression to the nonsegmented cephalic domain resembles the restricted forebrain expression pattern of its mammalian homologues. Lox10 is also expressed within the endodermal tissues of the leech midgut, which arises by cellularization from a polynucleate syncytium. Endodermal expression is organized into a pattern of transverse stripes and spots which are aligned with the intersegmental septa, and which prefigure the pattern of gut wall constrictions observed at later stages of development. Lox10 is the first molecular marker of segmentally periodic endoderm differentiation reported for any animal species.
Keywords
Amino Acid Sequence Animals Cell Differentiation Consensus Sequence Endoderm/*metabolism Ganglia, Autonomic/metabolism Gene Expression Regulation Genes, Helminth *Genes, Homeobox Invertebrates/genetics Leeches/embryology/*genetics/metabolism Mammals/genetics Molecular Sequence Data Morphogenesis *Multigene Family Nerve Tissue Proteins/biosynthesis/genetics Nervous System/*embryology/metabolism Phylogeny Prosencephalon/embryology Sequence Alignment Sequence Homology, Amino Acid Transcription Factors/biosynthesis/*genetics
Pubmed
Web of science
Create date
25/01/2008 14:35
Last modification date
20/08/2019 14:00
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