Influenza A Virus Induces Autophagosomal Targeting of Ribosomal Proteins

Details

Serval ID
serval:BIB_4C5CD59E1E29
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Influenza A Virus Induces Autophagosomal Targeting of Ribosomal Proteins
Journal
Mol Cell Proteomics
Author(s)
Becker A. C., Gannage M., Giese S., Hu Z., Abou-Eid S., Roubaty C., Paul P., Buhler L., Gretzmeier C., Dumit V. I., Kaeser-Pebernard S., Schwemmle M., Munz C., Dengjel J.
ISSN
1535-9484 (Electronic)
ISSN-L
1535-9476
Publication state
Published
Issued date
10/2018
Volume
17
Number
10
Pages
1909-1921
Language
english
Notes
Becker, Andrea C
Gannage, Monique
Giese, Sebastian
Hu, Zehan
Abou-Eid, Shadi
Roubaty, Carole
Paul, Petra
Buhler, Lea
Gretzmeier, Christine
Dumit, Veronica I
Kaeser-Pebernard, Stephanie
Schwemmle, Martin
Munz, Christian
Dengjel, Jorn
eng
Research Support, Non-U.S. Gov't
Mol Cell Proteomics. 2018 Oct;17(10):1909-1921. doi: 10.1074/mcp.RA117.000364. Epub 2018 Jul 6.
Abstract
Seasonal epidemics of influenza A virus are a major cause of severe illness and are of high socio-economic relevance. For the design of effective antiviral therapies, a detailed knowledge of pathways perturbed by virus infection is critical. We performed comprehensive expression and organellar proteomics experiments to study the cellular consequences of influenza A virus infection using three human epithelial cell lines derived from human lung carcinomas: A549, Calu-1 and NCI-H1299. As a common response, the type I interferon pathway was up-regulated upon infection. Interestingly, influenza A virus infection led to numerous cell line-specific responses affecting both protein abundance as well as subcellular localization. In A549 cells, the vesicular compartment appeared expanded after virus infection. The composition of autophagsomes was altered by targeting of ribosomes, viral mRNA and proteins to these double membrane vesicles. Thus, autophagy may support viral protein translation by promoting the clustering of the respective molecular machinery in autophagosomes in a cell line-dependent manner.
Keywords
Autophagosomes/*metabolism, Autophagy, Cell Line, Tumor, Humans, Influenza A virus/*metabolism, Influenza, Human/metabolism/pathology/virology, Proteome/metabolism, RNA, Messenger/genetics/metabolism, RNA, Viral/metabolism, Ribosomal Proteins/*metabolism, Ribosomes/metabolism, *Autophagy, *Cell biology, *Ribosomes, *silac, *Viruses, *autophagosome, *cell line, *organelle, *proteomics, *vesicle
Pubmed
Create date
10/03/2022 11:43
Last modification date
11/03/2022 7:33
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