Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_4BE5E0C98810
Type
Article: article from journal or magazin.
Collection
Publications
Title
Insect pathogenicity in plant-beneficial pseudomonads: phylogenetic distribution and comparative genomics.
Journal
The ISME Journal
Author(s)
Flury P., Aellen N., Ruffner B., Péchy-Tarr M., Fataar S., Metla Z., Dominguez-Ferreras A., Bloemberg G., Frey J., Goesmann A., Raaijmakers J.M., Duffy B., Höfte M., Blom J., Smits T.H., Keel C., Maurhofer M.
ISSN
1751-7370 (Electronic)
ISSN-L
1751-7362
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
10
Number
10
Pages
2527-2542
Language
english
Abstract
Bacteria of the genus Pseudomonas occupy diverse environments. The Pseudomonas fluorescens group is particularly well-known for its plant-beneficial properties including pathogen suppression. Recent observations that some strains of this group also cause lethal infections in insect larvae, however, point to a more versatile ecology of these bacteria. We show that 26 P. fluorescens group strains, isolated from three continents and covering three phylogenetically distinct sub-clades, exhibited different activities toward lepidopteran larvae, ranging from lethal to avirulent. All strains of sub-clade 1, which includes Pseudomonas chlororaphis and Pseudomonas protegens, were highly insecticidal regardless of their origin (animals, plants). Comparative genomics revealed that strains in this sub-clade possess specific traits allowing a switch between plant- and insect-associated lifestyles. We identified 90 genes unique to all highly insecticidal strains (sub-clade 1) and 117 genes common to all strains of sub-clade 1 and present in some moderately insecticidal strains of sub-clade 3. Mutational analysis of selected genes revealed the importance of chitinase C and phospholipase C in insect pathogenicity. The study provides insight into the genetic basis and phylogenetic distribution of traits defining insecticidal activity in plant-beneficial pseudomonads. Strains with potent dual activity against plant pathogens and herbivorous insects have great potential for use in integrated pest management for crops.

Pubmed
Web of science
Open Access
Yes
Create date
14/09/2015 8:24
Last modification date
20/08/2019 14:00
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