The rationale of retinal endovascular fibrinolysis in the treatment of retinal vein occlusion : from experimental data to clinical application.

Details

Serval ID
serval:BIB_4BC10B55910B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The rationale of retinal endovascular fibrinolysis in the treatment of retinal vein occlusion : from experimental data to clinical application.
Journal
Retina
Author(s)
Pournaras C.J., Petropoulos I.K., Pournaras J.A., Stangos A.N., Gilodi N., Rungger-Brändle E.
ISSN
1539-2864 (Electronic)
ISSN-L
0275-004X
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
32
Number
8
Pages
1566-1573
Language
english
Notes
Publication types: Journal Article
Abstract
PURPOSE: : We describe a retinal endovascular fibrinolysis technique to directly reperfuse experimentally occluded retinal veins using a simple micropipette.
METHODS: : Retinal vein occlusion was photochemically induced in 12 eyes of 12 minipigs: after intravenous injection of 10% fluorescein (1-mL bolus), the targeted retinal vein segment was exposed to thrombin (50 units) and to Argon laser (100-200 mW) through a pars plana approach. A beveled micropipette with a 30-μm-diameter sharp edge was used for micropuncture of the occluded vein and endovascular microinjection of tissue plasminogen activator (50 μg/mL) in 11 eyes. In one control eye, balanced salt solution was injected. The lesion site was examined histologically.
RESULTS: : Retinal vein occlusion was achieved in all cases. Endovascular microinjection of tissue plasminogen activator or balanced salt solution led to reperfusion of the occluded retinal vein in all cases. Indicative of successful reperfusion were the following: continuous endovascular flow, unaffected collateral circulation, no optic disk ischemia, and no venous wall bleeding. However, balanced salt solution injection was accompanied by thrombus formation at the punctured site, whereas no thrombus was observed with tissue plasminogen activator injection.
CONCLUSION: : Retinal endovascular fibrinolysis constitutes an efficient method of micropuncture and reperfusion of an experimentally occluded retinal vein. Thrombus formation at the punctured site can be prevented by injection of tissue plasminogen activator.
Pubmed
Web of science
Create date
11/10/2012 16:51
Last modification date
20/08/2019 13:59
Usage data