Positional correlative anatomy of invertebrate model organisms increases efficiency of TEM data production.
Details

UNIL restricted access
State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_4A98E0DD30CC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Positional correlative anatomy of invertebrate model organisms increases efficiency of TEM data production.
Journal
Microscopy and microanalysis
ISSN
1435-8115 (Electronic)
ISSN-L
1431-9276
Publication state
Published
Issued date
10/2014
Peer-reviewed
Oui
Volume
20
Number
5
Pages
1392-1403
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Transmission electron microscopy (TEM) is an important tool for studies in cell biology, and is essential to address research questions from bacteria to animals. Recent technological innovations have advanced the entire field of TEM, yet classical techniques still prevail for most present-day studies. Indeed, the majority of cell and developmental biology studies that use TEM do not require cutting-edge methodologies, but rather fast and efficient data generation. Although access to state-of-the-art equipment is frequently problematic, standard TEM microscopes are typically available, even in modest research facilities. However, a major unmet need in standard TEM is the ability to quickly prepare and orient a sample to identify a region of interest. Here, I provide a detailed step-by-step method for a positional correlative anatomy approach to flat-embedded samples. These modifications make the TEM preparation and analytic procedures faster and more straightforward, supporting a higher sampling rate. To illustrate the modified procedures, I provide numerous examples addressing research questions in Caenorhabditis elegans and Drosophila. This method can be equally applied to address questions of cell and developmental biology in other small multicellular model organisms.
Keywords
Animals, Caenorhabditis elegans/ultrastructure, Drosophila/ultrastructure, Microscopy, Electron, Transmission/methods
Pubmed
Web of science
Create date
01/10/2021 9:39
Last modification date
24/03/2025 19:05