Inhibition of cytolytic T lymphocyte clones reactive with Moloney leukemia virus-associated antigens by monoclonal antibodies: a direct approach to the study of H-2 restriction

Details

Serval ID
serval:BIB_4A6D2F58A1C7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Inhibition of cytolytic T lymphocyte clones reactive with Moloney leukemia virus-associated antigens by monoclonal antibodies: a direct approach to the study of H-2 restriction
Journal
Journal of Immunology
Author(s)
Weiss  A., MacDonald  H. R., Cerottini  J. C., Brunner  K. T.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
02/1981
Volume
126
Number
2
Pages
482-5
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
H-2 restriction in cytolytic T lymphocyte (CTL)-mediated lysis of syngeneic murine Moloney leukemia virus (MoLV)-induced tumor cells was studied at the clonal level by testing the inhibitory effect of monoclonal anti-H-2 antibodies on the lytic interaction between CTL clones and target cells. Large numbers of MoLV-specific CTL clones were generated by placing limiting numbers of C57BL/6 regressor (responder) spleen cells into micro-mixed leukocyte-tumor cell cultures. The clonal CTL populations thus obtained were split into 5 aliquots and tested for lytic activity in the presence (or absence) of 1 of 3 monoclonal antibodies or of an anti-whole H-2b haplotype antiserum. Two of the monoclonal antibodies were directed against H-2Db and one against H-2Kb determinants. Specificity of these reagents had been verified by demonstrating inhibition of lysis by CTL populations directed against H-2Db and H-2Kb alloantigens. In 44 of a total of 51 clones tested, results showed selective inhibition by the anti-H-2Db (and the anti-whole haplotype) reagents, and lack of inhibition by the anti-H-2Kb antibody., Of the remaining 7 clones, none was inhibited by the anti-H-2Db antibody, and 3 were inhibited by the anti-whole haplotype antiserum. These studies show that the recognition of MoLV-associated antigens by the majority of CTL clones was restricted to the H-2Db region, and that there exists limited heterogeneity in the H-2 restriction of such clones.
Keywords
Animals Antibodies, Neoplasm Antigen-Antibody Reactions *Antigens, Neoplasm Clone Cells/immunology *Cytotoxicity, Immunologic H-2 Antigens Leukemia, Experimental/*immunology Lymphocyte Culture Test, Mixed Mice Mice, Inbred C57BL Moloney murine leukemia virus/immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 13:58
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