Detection of Synaptic Proteins in Microglia by Flow Cytometry.
Details
Serval ID
serval:BIB_49FE862930E6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of Synaptic Proteins in Microglia by Flow Cytometry.
Journal
Frontiers in molecular neuroscience
ISSN
1662-5099 (Print)
ISSN-L
1662-5099
Publication state
Published
Issued date
2020
Peer-reviewed
Oui
Volume
13
Pages
149
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
A growing body of evidence indicates that microglia actively remove synapses in vivo, thereby playing a key role in synaptic refinement and modulation of brain connectivity. This phenomenon was mainly investigated in immunofluorescence staining and confocal microscopy. However, a quantification of synaptic material in microglia using these techniques is extremely time-consuming and labor-intensive. To address this issue, we aimed to quantify synaptic proteins in microglia using flow cytometry. With this approach, we first showed that microglia from the healthy adult mouse brain contain a detectable level of VGLUT1 protein. Next, we found more than two-fold increased VGLUT1 immunoreactivity in microglia from the developing brain (P15) as compared to adult microglia. These data indicate that microglia-mediated synaptic pruning mostly occurs during the brain developmental period. We then quantified the VGLUT1 staining in microglia in two transgenic models characterized by pathological microglia-mediated synaptic pruning. In the 5xFAD mouse model of Alzheimer's disease (AD) microglia exhibited a significant increase in VGLUT1 immunoreactivity before the onset of amyloid pathology. Moreover, conditional deletion of TDP-43 in microglia, which causes a hyper-phagocytic phenotype associated with synaptic loss, also resulted in increased VGLUT1 immunoreactivity within microglia. This work provides a quantitative assessment of synaptic proteins in microglia, under homeostasis, and in mouse models of disease.
Keywords
5xFAD model, TDP-43 conditional knock-out, VGLUT1, microglia, synaptic pruning
Pubmed
Web of science
Open Access
Yes
Create date
09/11/2020 9:18
Last modification date
30/04/2021 6:10